长程高脂饮食对实验大鼠糖尿病形成的影响  

Effects of Long-term High-fat Diet on the Occurrence of Diabetes in Rats

作　　者：殷峻[1] 陈名道[2] 周丽斌[2] 唐金凤[2] 李凤英[2] 陈家伦[2] 

机构地区：[1]上海第二医科大学附属新华医院内分泌科,上海200092 [2]上海第二医科大学附属瑞金医院,上海市内分泌研究所

出　　处：《中国医师杂志》2004年第1期47-49,共3页Journal of Chinese Physician

摘　　要：目的　研究长程高脂饮食及多食少动的现代生活方式对实验大鼠胰岛素抵抗和胰岛功能的影响。方法　Wistar大鼠75只喂以普通饲料或高脂饲料共 11月 ,部分高脂大鼠第 5月时注射 12mg/kg链脲霉素造成糖尿病模型。用腹腔注射糖耐量试验评估胰岛功能 ,胰岛素耐量试验检测胰岛素敏感性。结果　高脂大鼠糖负荷后 2h相血糖较普食鼠显著增高 ,第 11月时高脂鼠空腹血糖亦有增高。第 4月时 ,高脂鼠较普食鼠体重显著增加 ,胰岛素敏感性降低 5 0 %;此后二组胰岛素敏感性均有大幅下滑 ,第 9月时需用 0 5U/kg的胰岛素才能造成第 4月时 0 1U/kg胰岛素注射后的血糖降幅 ,此时高脂鼠的胰岛素敏感性与普食鼠已处于同一水平。结论　多食少动的现代生活方式是导致胰岛素抵抗的根本原因 。Objective To investigate the effects of long-term high-fat diet and the modern lifestyle of more food and lack of exercise on insulin resistance and islet function. Methods 75 Wistar rats were feed by routine or high-fat diet for 11 months. Some of high-fat rats were induced to diabetic models by injecting 12mg/kg streptozotocin. Islet function was evaluated by intraperitoneal glucose tolerance test. Insulin sensitivity was detected by insulin tolerance test. Results Compared with control group, 2h blood glucose of high-fat group was elevated significantly. Fasting blood glucose of high-fat group was also elevated at eleventh month. Body weight of high-fat group was increased remarkably compared with control group, and the insulin sensitivity of high-fat group was decreased by 50% at forth month. Then insulin sensitivity of both groups was declined greatly. Hypoglycemic effect of 0.5 U/kg insulin at ninth month was similar to that of 0.1U/kg insulin at forth month. Furthermore, no significant difference of insulin sensitivity was observed between high-fat group and control. Conclusions Main course for insulin resistance is the modern lifestyle of more food and lack of exercise. The main effect of high-fat diet on the occurrence of diabetes is damaging islet function.

关 键 词：高脂饮食 大鼠 糖尿病 胰岛素抵抗 动物模型 胰岛素耐量试验 

分 类 号：R587.1[医药卫生—内分泌]