小鼠腭突正常发育与腭裂形成中差异cDNA文库的建立  

A Subtractive cDNA Library of Gene Differentially Expressed during Development of Cleft Palate in Mouse.

作　　者：李鑫[1] 金岩[1] 岳文[2] 晏伟[3] 

机构地区：[1]第四军医大学口腔医学院病理科,陕西西安710032 [2]军事医学科学院野战输血研究所 [3]第四军医大学病理教研室

出　　处：《口腔医学研究》2003年第6期476-479,共4页Journal of Oral Science Research

摘　　要：目的 :建立小鼠腭突正常发育与腭裂形成中差异表达基因文库 ,揭示小鼠腭裂发生机制。方法 :利用C5 7BL/ 6N小鼠腭裂模型 ,直接提取正常组及RA处理组发育形成期腭突组织中的mRNA ,利用基于PCR的改良消减杂交技术 ,建立小鼠腭突正常发育时期与腭裂形成过程组织中差异表达基因文库。结果 :共获得阳性克隆 5 97个 ,蓝色克隆 16 8个 ,文库总浓度为 7.6× 10 2 。结论 :有效地构建高容量的腭裂发生过程中差异表达文库 。Objective:In order to reveal the mechanism of mouse cleft palate,the author established a subtractive cDNA library of gene differentially expressed in palatal process between normal group and RA treated group mouse.Methods:mRNAs were isolated respectively from palatal process in control and RA treated group C57BL/6N strain mouse on gestation day 12. The cDNA library was contructed by an improved technique based on PCR subtractive hybridization. Part of clones were randomly selected and identified with dot blot hybridization.Results:A subtractive cDNA library of cleft palate was constructed with a capacity of 7.6 ×10 2.Conclusions:To construct a efficient and high capacity subtractive cDNA library of cleft palate will be a forceful tool for identifying mechanism of cleft palate in gene level.

关 键 词：小鼠 腭突正常发育 腭裂 CDNA文库 基因表达 差异基因 

分 类 号：R782.22[医药卫生—口腔医学]