An armed oncolytic adenovirus system,ZD55-gene,demonstrating potent antitumoral efficacy  

作　　者：ZILAIZHANG WEIGUOZOU CHUNXIALUO BINGHUALI JINHUIWANG LANYINGSUN QIJUNQIAN XINYUANLIU 

机构地区：[1]LaboratoryofBiotechnology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [2]EasternHeptobiliaryHospital,SecondMilitaryMedicalUniversity,Shanghai200438,China

出　　处：《Cell Research》2003年第6期481-489,共9页细胞研究（英文版）

基　　金：supported by the Key Project of the Chinese Academy of Sciences(No.KSCX2-3-06);the National Natural Science Foundation of China(No.30120160823);the Chinese National“863”High Tech Project Foundation grant(No.2002AA216021).

摘　　要：ONYX-015 is an attractive therapeutic adenovirus for cancer because it can selectively replicate in tumor cells and kill them. To date, clinical trials of this adenovirus have demonstrated marked safety but not potent enough when it was used alone. In this paper, we put forward a novel concept of Gene-ViroTherapy strategy and in this way, we constructed an armed therapeutic oncolytic adenovirus system, ZD55-gene, which is not only deleted of ElB 55-kD gene similar to ONYX-015, but also armed with foreign antitumor gene. ZD55-gene exhibited similar cytopathic effects and replication kinetics to that of ONYX-015 in vitro. Importantly, the carried gene is expressed and the expression level can increase with the replication of virus. Consequently, a significant antitumoral efficacy was observed when ZD55-CD/5-FU was used as an example in nude mice with subcutaneous human SW620 colon cancer. Our data demonstrated that ZD55-gene, which utilizing the Gene-ViroTherapy strategy, is more efficacious than each individual component in vivo.

关 键 词：ZD55-gene oncolytic adenovirus ElB 55-kD gene cancer Gene-ViroTherapy. 

分 类 号：R730.54[医药卫生—肿瘤]