Mortalin imaging in normal and cancer cells with quantum dot immuno conjugates  

作　　者：ZEENIA KAUL TOMOKO YAGUCHI SUNIL C KAUL TAKASHI HIRANO RENU WADHWA KAZUNARI TAIRA 

机构地区：[1]National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan ,National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan ,National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan ,National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan ,National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan ,National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki3058562, Japan

出　　处：《Cell Research》2003年第6期503-507,共5页细胞研究（英文版）

摘　　要：Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine,and have several predictive advantages.These include their i)broad absorption spectra allowing visualization with single light source,ii)exceptional photo-stability allowing long term studies and iii)narrow and symmetrical emission spectrum that is controlled by their size and material composition.These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source,their simultaneous resolution and visualization as different colors.At present there are only a few studies that have tested quantum dots in cellular imaging.We describe here the use of quantum dots in mortalin imaging of normal and cancer cells.Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.

关 键 词：量子 正常细胞 癌细胞 免疫 

分 类 号：R730.4[医药卫生—肿瘤]