精氨酸增加大鼠海马脑片可溶性淀粉样前体蛋白的释放  

Effect of L-arginine on release of soluble amyloid precursor protein from rat hippocampal brain slices

作　　者：张癸荣[1] 高登莲[2] 曹龙光[3] 殷明[3] 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [2]中国人民解放军第三零二医院,北京100039 [3]上海交通大学药学院,上海200030

出　　处：《天津药学》2003年第6期1-4,共4页Tianjin Pharmacy

基　　金：国家自然科学基金资助项目 (No.39770 80 7)

摘　　要：目的 :观察兴奋性神经递质精氨酸对于可溶性淀粉样前体蛋白 (s APP)释放的影响。方法 :雄性 SD大鼠断头取海马后于 0～ 4 o C Krebs- Ringer液中快速振动切片 ,切片于 37o C预孵育后置含精氨酸或不含精氨酸 Krebs- Ringer液(对照 )中孵育后于 15 0 0 0 g离心取上清液 ,应用二辛可宁酸法测定上清液总蛋白含量 ;释放入孵育液中的 s APP以Western blot方法测定。结果 :正常孵育的海马脑片能够分泌基础量的 s APP,精氨酸刺激能够导致海马脑片释放 s APP增加 ,但是没有导致新蛋白的产生。结论 :精氨酸在 10 - 9～ 10 μmol/ L浓度范围内 ,均能够导致海马脑片分泌 sObject: To observe the effects of excitatory neurotransmitter L-arginine on the release of the soluble amyloid precursor protein (sAPP). Methods: Male Sprague-Dawley rats were decapitated and hippocampus were dissected at 4℃ , then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at 0~4℃ by a vibration chopper. After the slices were preincubated in Krebs-Ringer buffer at 37℃, they were stimulated with various concentrations of L-arginine in Krebs-Ringer buffer. The supernatant were removed from the solution after centrifugation at 15 000 g. Contents of total protein were measured by bicinchoninic acid assay (BCA). The sAPP derivates released into the incubation medium were assayed by Western blot analysis. Results: L-arginine has increased the release of the soluble amyloid precursor protein (sAPP) in incubated hippocampal brain slices. However, failed to produce special protein. Conclusion: L-arginine has positive effects on the release of the soluble amyloid precursor protein (sAPP) from concentration of 10 -15 mol/L to 10 -5 mol/L.

关 键 词：精氨酸 可溶性淀粉样前体蛋白 阿尔茨海默病 海马脑片 WESTERN BLOT 大鼠 AD 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R977.4[医药卫生—临床医学]