外周血淋巴细胞与癌细胞化疗药物敏感性试验在肝癌患者中的应用  

Application of medicine sensitivity test of chemotherapeutics agents in peripheral blood lymphocyte and cancer cell with hepatocarcinoma patients

作　　者：皇甫超申[1] 王玉兰[2] 

机构地区：[1]河南大学医学院病理教研室,河南开封475001 [2]河南大学医学院医学系,河南开封475001

出　　处：《临床荟萃》2004年第2期79-80,共2页Clinical Focus

基　　金：河南省科技攻关项目 (编号 :1 2 4 1 70 2 2 9)

摘　　要：目的　利用肝癌患者外周血淋巴细胞代替癌细胞进行化疗药物敏感性测定 ,指导个体化治疗。方法　取肝癌标本的同时抽取其外周血 ,利用噻唑蓝 (MTT)法 ,体外检测 2 6例患者癌细胞和淋巴细胞对 8种化疗药物的敏感性。结果　肝癌患者癌细胞抑制率与外周血淋巴细胞抑制率间呈正相关关系。结论　肝癌患者外周血淋巴细胞化疗药物敏感性检测对临床选择化疗药物具有重要参考价值。Objective The medicine sensitivity test of chemotherapeutic agents had been undergone by using peripheral blood lymphocyte instead of cancer cell in patients with hepatocarcinoma to direct individual chemotherapy. Methods To phlebotomize while getting liver canner specimen, by using a tetrazolium based colorimetrie assay(MTT assay),the chemosensitivity of lymphocyte and cancer cell in 26 patients with liver cancer to 8 chemotherapentic agents were detected in vitro. Results The inhibited rate between cancer cell and lymphocyte showed positive correlation. Conclusion Medicine sensitivity test of the peripheral blood lymphocyte is of important reference value to choose chemotherapeutic drugs in hepatocarcinoma patients.

关 键 词：淋巴细胞 药物筛选试验 外周血 癌细胞 药物敏感性 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R73-361[医药卫生—临床医学]