脐血、健康人及慢粒患者外周血来源的DC介导的抗白血病免疫效应研究  

STUDY ON THE ANTI-LEUKEMIA IMMUNE EFFECT INDUCED BY DENDRITIC CELLS DERIVED FROM CORD BLOOD,NORMAL HUMAN PERIPHERAL BLOOD AND CHRONIC MYELOID LEUKEMIA CELLS

作　　者：宋飞雪[1] 张连生[1] 张筠[1] 

机构地区：[1]兰州医学院第二附属医院,甘肃兰州730030

出　　处：《白血病．淋巴瘤》2003年第6期333-337,共5页Journal of Leukemia & Lymphoma

基　　金：甘肃省科学事业费资助项目 ( QS0 0 2 -C3 3 -19)

摘　　要：目的 :观察脐血、健康人及慢性粒细胞性白血病 (CML )患者外周血 3种不同来源的树突状细胞 (DC)及其抗白血病效应。方法 :分离 3种来源的单个核细胞 (MNC)并在细胞因子 (GM- CSF+IL - 4 +TNF-α)作用下诱导培养 DC。观察 DC形态 ,流式细胞技术检测 DC免疫表型 (CD1 a、CD80 、CD83、HL A- DR) ;将所得 DC经 CML细胞可溶性抗原负载后和其同源 T细胞混合诱导抗原特异性CTL,MTT法检测其杀伤活性。结果 :3种来源 MNC在细胞因子作用下均可获得形态典型的 DC,且高表达 CD1 a、CD80 、CD83、HL A- DR,经抗原负载后的 DC诱导的 CTL 对 CML 靶细胞的杀伤活性也较强。结论 :脐血、健康人及 CML 患者外周血均为理想的 DC来源 ;有较好的诱导 CTL 杀伤白血病细胞效应 ;应用 DC瘤苗免疫治疗白血病必将成为一种崭新的肿瘤免疫治疗方法 ,有十分诱人的发展前景。Objective:In the project, the condition to induce DCs from cord blood, normal human peripheral blood and chronic myeloid leukemia (CML) cells and their anti leukemia response were investigated.Methods:The mononuclear cells were cultured with the cytokines (GM CSF+IL 4+TNF α) for inducing DC. The morphologic features were analyzed with optical microscopy and the phenotype of DC was detected by FACS with CD 1a , CD 80 , CD 83 and HLA DR monoclonal antibodies. DCs were pulsed by the CML cell lysates and incubated with autologous T lymphocytes for 5d to induce antigen specific CTL. Cytotoxicity assay was measured by MTT method.Results:It was proved that the DCs derived from the different sources showed a typical morphology of DC. A high expression of phenotypes was found in CB DC, PB DC and CML DC stimulated by the cytokines. The antigen loaded DCs from different sources, all of them have strong capacity of stimulating CTL reaction.Conclusion:It is concluded that cord blood, normal human and CML patients peripheral blood cells could successfully induce functional DCs; They have the ability of inducing CTL reactionto clear leukemia cells. It is a novel idea that expanding DC in vitro and enhancing their antitumor immunefunction and DC based tumor vaccines may be used as an efficient immune therapy for leukemia and others.

关 键 词：树突状细胞 脐血 慢性粒细胞白血病 外周血 免疫学 

分 类 号：R733.72[医药卫生—肿瘤]