Ralstonia metallidurans CH34苯酚、甲苯降解基因的生物信息学分析  

Analysis of genes involved in degradation of phenol, toluene in Ralstonia metallidurans CH34 genome by means of bioinformatics

作　　者：宋水山[1] 段普凡[1] 贾振华[1] 高振贤[1] 马宏[1] 张小兵[1] 

机构地区：[1]河北省科学院生物研究所,石家庄050081

出　　处：《河北省科学院学报》2003年第4期245-251,262,共8页Journal of The Hebei Academy of Sciences

摘　　要：　采用生物信息学方法对RalstoniametalliduransCH34基因组序列进行了深入分析,推测了参与降解苯酚、甲苯/二甲苯降解的有关基因及功能,编码序列推测结果显示,R.metalliduransCH34中可能存在苯酚、甲苯/二甲苯间位降解代谢途径的基因操纵子及其调节因子和辅助因子,为后续进一步克隆和研究各基因的准确功能提供了有益的信息,奠定了基础。Nucleotide sequence of Ralstonia metallidurans CH34 genome has been mined with respect to the genes probably involved in degradation of phenol or toluene by means of bioinformatics. Homology analysis of gene sequence and their deduced amino acid sequence revealed that a large gene operon including all structure, regulatory and facilitator genes required for degradation of phenol or toluene via meta-cleavage pathway was most likely present in the genome of Ralstonia metalliduransCH34. A positive cascade regulatory mechanism probably existed in R. metallidurans CH34. The results reported here offered a base for fully understanding the molecular mechanism of degradation of phenol or toluene by R. metallidurans CH34.

关 键 词：Ralstonia metallidurans CH34 苯酚 甲苯 降解 基因 生物信息学 革兰氏阴性菌 废水处理 

分 类 号：X172[环境科学与工程—环境科学] Q78[生物学—分子生物学]