人外周血单核细胞来源的DC体外分化成熟影响因素的研究  

The Affecting Factors of Inducing Human Peripheral Blood Monocyte-Derived Dendritic Cells in vitro

作　　者：束永前[1] 朱一蓓[2] 戴俊[2] 席弘[2] 黄勇[2] 吴明媛[2] 夏瑜[2] 张学光[2] 

机构地区：[1]江苏省人民医院肿瘤中心,南京210029 [2]苏州大学医学生物技术研究所,江苏苏州215007

出　　处：《中国肿瘤生物治疗杂志》2003年第4期243-247,共5页Chinese Journal of Cancer Biotherapy

基　　金：江苏省医学重点实验室;江苏省卫生厅科研基金(H20010);江苏省卫生厅科技重大攻关项目(H200211)

摘　　要：目的:探讨不同因素对人外周血单核细胞来源的树突状细胞(DC)体外分化成熟的影响。方法:通过流式细胞仪表型分析、混合淋巴细胞反应、抗原摄取能力检测、DC对T细胞的趋化能力及对IL-10的拮抗效应的测定,研究了TNF-α,FL,sCD40L,CD40mAb,gp130,IL-10等不同因素对DC的体外分化成熟及功能的影响。结果;CD40信号和TNF-α都可以有效地使DC上调表达共刺激分子CD80,CD86并进而促进DC激发T细胞的增殖和活化及对T淋巴细胞的趋化作用,但在体外培养体系中,sCD40L能有效地站抗IL-10的抑制效应,而TNF-α则明显不如sCD40L有效;激发型gp130单克隆抗体和人重组FL可以显著地促进DC的体外扩增,但是无明显地促进DC分化成熟和促进DC激发T细胞的功能。结论:CD40和TNF-α都具有促进DC分化、成熟的能力,但CD40的作用明显优于TNF-α。Objective: To study the affecting factors on the differentiation, maturation and function on the human pe- ripheral blood monocyte-derived dendritic cells (DC). Methods: Through DCs' surface molecules analysis, mixed-lym- phocyte reaction, FITC-Dextran capture, cell counting and chemotaxis activity of T lymphocytes, we compared functions of DC stimulated with different cytokines including TNF-α, FL, sCD40L, CD40mAb, gp130 and IL-10. Results: Both CD40- and TNF-α signalling are able to promote differentiation and maturation of DC, including upregulation of costimula- tory molecules expression, such as CD80 and CD86, enhancement of DC-mediated MLR, promotion of T cell chemotactic ability to DC; CD40 signalling counteracted the inhibitory effects of IL-10 on DC more efficiently than TNF-α signalling; The agonist gp130 mAb and human recombinant FL can remarkably promote the proliferation of DC in vitro, while there are no distinct effects on DCs' differentiation, maturation and ability to activate T cells. Conclusions: CD40 signalling is more powerful than TNF-α, and plays a unique role to abtain more mature and functional DC in vitro.

关 键 词：树突状细胞 体外诱导 共刺激分子 CD40 TNF-α 免疫应答 肿瘤免疫 

分 类 号：R730.3[医药卫生—肿瘤]