高温胁迫对Bt转基因棉叶片毒蛋白含量的影响  

EFFECTS OF HEAT STRESS ON TOXIN PROTEIN CONTENT IN Bt TRANSGENIC COTTON LEAVES

作　　者：周桂生[1] 封超年[1] 周青[2] 张网定 陈后庆[1] 

机构地区：[1]扬州大学农学院农学系,江苏扬州225009 [2]淮阴工学院农业与食品工程系,江苏淮阴223001 [3]江苏省扬州市气象局,江苏扬州225009

出　　处：《江苏农学院学报》2003年第4期75-77,共3页Jiangsu Agricultural Research

基　　金：江苏省高校自然科学基金资助项目(011CJB210002)

摘　　要：以科棉1号为试验材料,研究连续48 h的40℃高温胁迫对棉花果枝叶片游离氨基酸、可溶性蛋白和Bt毒蛋白含量的影响。结果表明:高温胁迫后叶片游离氨基酸含量一直增加,其中以胁迫后12 h内增加速度最快;可溶性蛋白含量急剧下降,胁迫后24 h内下降最快;叶片Bt毒蛋白含量的变化趋势与可溶性蛋白基本一致。高温胁迫后可溶性蛋白含量与Bt毒蛋白含量呈极显著正相关,游离氨基酸含量则与Bt毒蛋白含量呈极显著负相关。与盛花期相比,结铃盛期高温胁迫对Bt毒蛋白含量的影响更大。With Bt transgenic cotton Kemian 1 as the testing material, the content of free amino acid , soluble protein and Bt toxin protein in fruit branch leaves were studied under the heat stress (40 ℃) which lasted for 48 h. The main results were as follows: The content of free amino acid presented a gradual increase under heat stress, showing a rapid increment during the first 12 h, while the content of soluble protein decreased, presenting a sharp decrement during the first 24 h. Heat stress reduced Bt toxin protein content in fruit branch leaves. Compared with full blossom period, heat stress produced a more profound effect on the Bt toxin protein content during boll developing period.

关 键 词：高温胁迫 Bt转基因棉 叶片 BT毒蛋白 游离氨基酸 可溶性蛋白 含量 

分 类 号：S562[农业科学—作物学]