一个马铃薯X病毒分离物的外壳蛋白基因序列分析与株系鉴定  

Coat protein gene analysis and identification of an isolate of potato virus X

作　　者：曲静[1] 朱常香[1] 温孚江[1] 郭兴启[1] 宋云枝[1] 

机构地区：[1]山东农业大学生命科学院,泰安271018

出　　处：《植物保护学报》2003年第4期358-364,共7页Journal of Plant Protection

基　　金：国家自然科学基金(39970485);山东省自然科学基金(22000D_(02))

摘　　要：对山东省侵染马铃薯的一个马铃薯X病毒(PVX)分离物PVX-SD_1的外壳蛋白(CP)基因进行了克隆和序列分析。以提纯的病毒RNA为模板,应用RTPCR扩增目的基因,通过常规的基因克隆法将扩增的CP基因导入pUC19载体,测序。结果表明,PVX-SD_1的CP基因长719bp,可编码248个氨基酸;与Gen-Bank中报道的15个有代表性的株系或分离物相比较,核苷酸同源性在80.1％～99.7％,氨基酸同源性在89.8％～100％;与欧洲株系UK_3仅1个核苷酸不同,同源性为99.7％,氨基酸同源性达100％,表明它们可能为同一株系,属于X^3细。An isolate of potato virus X ( PVX-SD;) was obtained from potato plants cultivated in Shandong Province. The coat protein ( CP) gene of PVX was amplified from the extracted viral RNA by using reverse transcription -poly merase chain reaction (RT-PCR) , and cloned into plasmid pUC19. The nucleotide sequence of the CP gene was determined and compared with other isolates reported in GenBank. It shares 80. 1% -99. 7% and 89. 8% - 100% homology with the reported isolates at the sequence levels of nucleotide and deduced amino acid, respectively. PVX-SD1 shares 99. 7% and 100% homologies at the nucleotide and deduced amino acid levels, respectively, with one of the European strain UK3, indicating that they could belong to the same strain.

关 键 词：马铃薯 X病毒分离物 外壳蛋白基因 序列分析 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]