影响肿瘤细胞核DNA含量分析的若干因素  

FACTORS AFFECTING THE RESULTS OF NUCLEAR DNA CONTENT ANALYSIS IN TUMOR CELLS

作　　者：应明[1] 印红霞[2] 陈长玉[1] 方振东[1] 

机构地区：[1]解放军第81医院骨科,南京210002 [2]南京军区南京总医院神经外科实验室,南京210002

出　　处：《临床肿瘤学杂志》1996年第1期19-21,共3页Chinese Clinical Oncology

摘　　要：采用静态细胞分析法(图像分析)和流式细胞分析法比较研究了影响肿瘤细胞核DNA含量分析正确结果的3种因素:分析方法、流式细胞分析所用的标本和同一肿瘤不同部位取材作流式细胞分析。结果表明:(1)同一肿瘤静态细胞分析与流式细胞分析DNA指数相关性好,但流式细胞分析DNA可能会遗漏接近二倍体的DNA异常。(2)同一肿瘤冷冻标本和石蜡标本分别行流式细胞分析,其DNA指数相关性良好,S期细胞百分数相关性稍差,用冷冻标本行流式细胞分析有较石蜡标本高的DNA异倍体检出率。(3)同一肿瘤不同部位取材DNA倍体水平结果相似,但DNA指数有差异。肿瘤细胞DNA含量异质性并不严重影响DNA异倍体的检出。From sample collection to results explanation there were many factors that could affect the conclusion of nuclear DNA content analysis in tumor cells. Among them, three factors as follow were investigated by static cytometry (SCM) and flow cytometry (FCM): analytic methods, different preparations of sample in FCM and different samples obtained from varied sites of the same tumor in FCM. The results showed: (l)The DNA index provided by SCM and FCM had a good correlation. However, FCM might miss the DNA content abnomality near diploid; (2)The DNA index provided by FCM used frozen sample and formarrin-enbeded one had a good correlation, and the s-phase fractions of the same tumor were some different. It was easier to detect the aneuploid of DNA if the sample was frozen in FCM; (3)The DNA ploid levels of different samples obtained from the same tumor were similar but their DNA indexes might have difference. The hetero-genecity of DNA in a tumor had little affection to the detection of DNA aneuploidy.

关 键 词：肿瘤 细胞核 DNA 流式细胞分析 图像分析 

分 类 号：R730[医药卫生—肿瘤]