细胞凋亡相关基因在膀胱移行细胞癌中表达的意义  

THE EXPRESSIONS OF P53、BCL2 AND BAX IN TRANSITIONAL CELL CARCINOMA OF BLADDER

作　　者：汪东[1] 夏养志[2] 阎俊[3] 

机构地区：[1]内蒙古自治区医院泌尿外科,内蒙古呼和浩特010017 [2]内蒙古医学院病理教研室 [3]内蒙古医学院第一附医院泌尿外科

出　　处：《内蒙古医学院学报》2003年第4期226-230,共5页Acta Academiae Medicinae Neimongol

摘　　要：目的 :探讨与细胞凋亡关系最为密切的三个基因 P5 3、BCL2及 BAX在膀胱移行细胞癌中的表达与其病理分级及预后的关系。方法 :采用 P5 3、BCL2单克隆抗体及 BAX多克隆抗体 ,对 70例膀胱移行细胞癌和 1 0例膀胱炎石蜡切片进行免疫组织化学染色。结果 :P5 3蛋白的阳性表达率在各级别间及复发与未复发组间的差异有统计学意义 ( P <0 .0 5或 P <0 .0 1 ) ;BCL2在 级肿瘤的阳性表达率 ( 4 4.4% )明显高于 级 ( 3 .5 % )的表达率 ( P <0 .0 5 ) ;BAX的阳性表达率在 级 ( 2 2 .2 % )则明显低于 ( 86.2 % )、 级 ( 73 .9% )的表达率 ( P <0 .0 5 ) ,BAX的阳性表达率在膀胱良恶性病变间无明显差异 ( P >0 .0 5 ) ;二者在复发组的阳性表达率明显高于未复发组 ( P <0 .0 5 ) ;BCL2 /BAX及与 P5 3三者的共同表达情况与肿瘤的恶性程度呈正相关 (均 P <0 .0 5 ) ,与预后无相关性 ( P >0 .0 5 )。结论 :P5 3、BCL2及 BAX的阳性表达可以在一定程度上反映膀胱移行细胞癌恶性程度的增加及临床预后不良 ,三者可能通过对细胞凋亡的调节从而影响肿瘤恶性性状的发展。Objective: To investigate the expression of P53、BCL2 and BAX in 70 transitional cell carcinomata of bladder (TCCB) and 10 cystitis. Methods: P53、BCL2 and BAX expression were assayed by means of immunohistochemical S-P method. Results: The positive rate of P53 protein expression was related to grade and prognosis (P<0.05 or P<0.01); There was statistically significant difference between grade Ⅰ tumor and grade Ⅲ for the positive rate of BCL2 protein expression (P<0.01). The difference between grade Ⅲ andⅠ、Ⅱ was significant for the expression of BAX protein. The expression of BCL2 and BAX were related to the tumor prognosis (P<0.05). The common expression of BCL2/BAX or P53、BCL2/BAX were corelated to grade of tumor (P<0.05). Conclusion: Expression of P53、BCL2 and BAX is a helpful index in predicating grade and prognosis of TCCB, and the progression of TCCB can be related to P53、BCL2 and BAX apoptosis function to tumor cells.

关 键 词：膀胱移行细胞癌 细胞凋亡 P53 BCL2 BAX 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]