原发性肝细胞癌中PTTG和c-myc基因表达的研究  

Expression of PTTG and c-myc gene in human primary hepatocellular carcinoma

作　　者：金中元[1] 程瑞雪[1] 郑长黎[1] 郑晖[1] 

机构地区：[1]中南大学湘雅医学院病理学教研室,湖南长沙市410078

出　　处：《世界华人消化杂志》2003年第11期1677-1681,共5页World Chinese Journal of Digestology

摘　　要：目的:研究 PTTG 和 c-myc 基因表达在原发性肝细胞癌 (HCC)发生发展中的作用.方法:应用原位杂交(DNA-RNA)技术与免疫组化 SP 法分别检测61例原发性肝细胞癌及癌旁肝组织中 PTTG mRNA 和PTTG 蛋白及 c-myc mRNA 和 c-myc 蛋白的表达.结果:在原发性肝细胞癌(HCC)中,PTTG mRNA 和 PTTG蛋白阳性细胞呈弥漫性、小巢状或散在分布,在胞质内呈全浆型、膜下型表达.PTTG mRNA 和 PTTG 蛋白在 HCC 中表达率分别为72.1%(44/61)和78.7%(48/61),在癌旁肝组织中分别为93.4%(57/61)和91.8%(56/61),在 HCC中表达明显低于癌旁肝组织(P<0.005,P<0.05).相关性检验显示癌及癌旁 PTTG 基因表达与 c-myc 基因表达呈正相关(P<0.005).结论:PTTG 基因过度表达参与了肝细胞癌发生发展,过度表达的 FTTG 可能通过激活癌基因 c-myc 来参与肝细胞恶性转化和肝细胞癌的发生发展过程.AIM:To investigate the relationship between expressions of PTTG and c-myc genes and oncogenesis in human hepa- tocellular carcinomas(HCC). METHODS:In situ hybridization(DNA-RNA)and immuno- histochemistry(SP method)methods were used to detect the expressions of PTTG and c-myc genes in 61 cases of human hepatocellular carcinomas. RESULTS:The distributions of positive cells of PTTG mRNA and PTTG protein in HCC were diffuse,aggregate or scattered.The positive stainings of PTTG mRNA and PTTG protein were plasma and submembrane types.The positive rates of PTTG mRNA and PTTG protein were 72.1%(44/61) and 78.7%(48/61) in HCC,and 93.4%(57/61)and 91.8% (56/61)in pericarcinomatous liver tissues.The expressions of PTTG mRNA and PTTG protein in HCC were significantly lower than those in paracancerous tissues(P<0.005,P<0.05). The expression of PTTG gene was significantly correlated With that of c-myc gene(P<0.005). CONCLUSION:Overexpression of PTTG is related to human hepatocellular carcinogenesis.C-myc gene activated by PTTG protein may play an important role in hepatocellular transformation and carcinogenesis.

关 键 词：原发性肝细胞癌 PTTG基因 C-MYC基因 原位杂交技术 免疫组化SP法 肿瘤发生 

分 类 号：R735.7[医药卫生—肿瘤]