大鼠胃黏膜损伤修复时早期应答基因c-Jun 及c-met的表达  

c-met and c-Jun expression during acute gastric mucosal lesions in rats

作　　者：姚永莉[1] 徐波[2] 宋于刚[1] 张万岱[1] 

机构地区：[1]中国人民解放军第一军医大学南方医院全军消化病研究所,广东省广州市510515 [2]中国人民解放军第一军医大学南方医院侨科,广东省广州市510515

出　　处：《世界华人消化杂志》2003年第11期1711-1714,共4页World Chinese Journal of Digestology

基　　金：军队"九五"卫生科研基金课题;No.96M060

摘　　要：目的:建立无水乙醇性胃黏膜损伤大鼠模型并观察早期应答基因 c-Jun 和 c-met 对胃黏膜损伤修复的影响.方法:采用无水乙醇1mL 胃饲诱发急性胃黏膜损伤大鼠模型,并于伤后0,4,8d 分别处理一组大鼠,观察损伤模型自然修复过程,免疫组织化学技术检测早期应答基因 c-Jun和 c-met 的表达.结果:在损伤模型自然修复过程中,损伤后4,8d 组大鼠胃黏膜损伤指数(LI)为32±7,18±3,均显著低于损伤模型组75±11,(P<0.05);免疫组织化学显示损伤后8d 组大鼠胃黏膜 c-Jun 的阳性表达率为87.5%,显著高于正常对照组的12.5%,c-met 的阳性表达率为62.5%,均显著高于正常对照的0及损伤模型组的0(P<0.05).结论:早期应答基因 c-Jun 和 c-met 的表达能促进急性胃黏膜损伤的修复,对黏膜损伤的自愈具有重要作用.AIM:To establish an ethanol-induced acute gastric mucosal lesions model in rats and to investigate the effect of c-met and c-Jun protein on healing of acute gastric mucosal lesions. METHODS:Animal models of acute gastric mucosal le- sions were established by intragastric instillation of 1 mL ethanol in the rats.On day 0,4,8,the rats were sacrificed respectively.Rats without any treatment served as control. The expressions of c-met and c-Jun were analyzed by im- munohistochemical staining. RESULTS:Gastric mucosal lesion indexes(LIs)were sig- nificantly lower after 4,and 8 days with acute gastric mucosal lesions(32±7,18±3)than LI of acute gastric mucosal lesion model rats(75±11)(P<0.05).Immunohistochemical staining revealed higher positive staining of c-Jun after 8 days with acute gastric mucosal lesions(87.5%)than those of normal control rats(12.5%),and higher positive staining of c-met after 8 days with acute gastric mucosal lesions(62.5%) than those of normal control(0)and acute gastric mucosal lesions model rats(0)(P<0.05). CONCLUSION: The expression of c-met and c-Jun could accelerate the healing of acute gastric mucosal lesions,which is important for the healing of acute gastric mucosal lesions.

关 键 词：大鼠 胃黏膜损伤 应答基因 C-JUN基因 C-MET基因 免疫组织化学 细胞增生 

分 类 号：R573[医药卫生—消化系统]