成年大鼠脑梗死后自体神经干细胞的原位增殖  

Experimental study on the proliferation of inherent neural stem cells in situ in the adult rats after cerebral infarction

作　　者：张波[1] 王任直[1] 李桂林[1] 姚勇[1] 窦万臣[1] 孔燕国[1] 田士强[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院神经外科,北京100730

出　　处：《基础医学与临床》2003年第6期616-619,共4页Basic and Clinical Medicine

基　　金：科技部重大基础研究前期研究专项研究基金 (2 0 0 2CCA0 4 4 0 0 )

摘　　要：制作大鼠脑梗死模型 ,采用免疫组化染色方法 ,观察脑梗死后 1、3、7、14和 2 8d时 ,梗死侧神经干细胞增殖及其表达标记物BrdU和nestin的动态变化。与对照组相比 ,伤侧皮层、海马及室下区的BrdU和nestin阳性细胞数于伤后 1d开始增多 ,7d达高峰 ,2 8d后接近正常水平。结果表明 :脑梗死可激发成年大鼠自体神经干细胞原位增殖 ;自体神经干细胞对脑梗死损伤有一定的反应能力 。To investigate the proliferation of inherent neural stem cells in situ in the adult rats after cerebral infarction cerebral infarction models of rats were established and the dynamic expression of bromodeoxyuridine(BrdU) and neuroepthelial stem cell protein (Nestin) were studied by immunohistochemistry staining. BrdU was used to mark dividing neural stem cells. Nestin was used to identify neural stem cells. Compared with the controls, the number of BrdU-positive and Nestin -positive cells in the subventricular zone,hippocampus and injured cortex markedly increased on 1st day, reached maximum on 7th day, markedly decreased on 14th day,but it was still elevated as compared with that of the controls, and remained unchanged at 28th day. The results indicate that cerebral infarction stimulated the proliferation of inherent neural stem cells in situ. these cells then respond to brain infarction and have potential therapeutic effect on self-recovery of brain infarction.

关 键 词：成年大鼠 脑梗死 自体神经干细胞 细胞原位增殖 动物模型 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R-33[医药卫生—临床医学]