利用小麦微卫星引物建立偃麦草E^e染色体组特异SSR标记  

Development of Specific SSR Marker for E^e-genome of Thinopyrum sp.by Using Wheat Microsatellites

作　　者：尤明山[1] 李保云[1] 田志会[2] 唐朝晖[1] 刘守斌[1] 刘广田[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]北京农学院园林系,北京102206

出　　处：《农业生物技术学报》2003年第6期577-581,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金重点项目(39910110);北京市自然科学基金重大项目(6990001)。

摘　　要：选用40对小麦 SSR 引物对17份偃麦草(Thinopyrum sp.)、2份小麦(Triticum aestivum)材料进行了 PCR 扩增分析,从中筛选到引物 Xgwm325能在不同偃麦草材料中扩增出4条长度分别为1400、440、120和100bp 的特异 DNA 片段,可以作为偃麦草种质的特异 SSR 标记。利用小麦-二倍体长穗偃麦草(Th.elongatum)异代换系和异附加系对引物 Xgwm 325进行了扩增鉴定,结果只有100bp 左右的片段出现在长穗偃麦草所有 E^e 组染色体上,该片段可以作为 E^e 染色体组的特异 SSR标记。Because of high morphology revealing capability and simpleness in operation,simple sequence repeats (SSR) marker is a very useful tool for genetic mapping,genetic diversity analysis and marker-assistant-selection; only the isolation of microsatellite DNA is a costly matter.One of the solutions for this problem is to use microsatellites to relative species.The subject of this study was to evaluate the possibility of transfering wheat microsatellites to Thinopyrum species,and to develop specific SSR markers for Thinopyrum germplasm by conducting PCR program with wheat SSR primers to 17 accessions of Thinopyrum species and 2 common wheat (Triticum aestivum) cultivars.Among the used 40 wheat SSR primers,25 pairs amplified PCR products in most of the alien species.And moreover,one pair primers,namely Xgwm325,could amplify four identical DNA fragment specific for Thinopyrum species in approximately length of 1400,440,120 and 100 bp respectively.These fragments could be used as specific SSR markers for Thinopyrum germplasm.To confirm the correctness of these molecular markers,a further PCR program with primer Xgwm325 was conducted to a full set of wheat-Th.elongatum alien disomic addition lines and 15 alien disomic substitution lines.The result showed that only the 100 bp fragment had been appeared in all alien chromosome possessed materials,and indicated that this fragment could be a molecular marker for entire genome of Th.elongatum,despite of the chromosome specificity of SSR markers in wheat.

关 键 词：小麦 微卫星引物 偃麦草 染色体组 SSR标记 

分 类 号：S512.1[农业科学—作物学]