硝酸镧长期灌胃大鼠肝脏中HSP70的表达  

Expression of HSP70 in Rat Livers after Long-term Administration Lanthanum Nitrate

作　　者：刘颖[1] 陈东[1] 陈爱军[1] 聂毓秀[1] 

机构地区：[1]吉林大学基础医学院组织学与胚胎学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2003年第6期727-728,共2页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (2 9890 2 80 - 3)

摘　　要：目的 :检测稀土化合物硝酸镧 [La( NO3 ) 3 ]经消化道长期作用 ,大鼠肝脏中热休克蛋白 70( HSP70 )的表达水平。方法 :以大鼠为研究对象 ,连续用生理盐水和 0 .1、0 .2、2 .0、1 0 .0及2 0 .0 mg· kg-1体重的 La( NO3 ) 3 灌胃 6个月后 ,应用免疫组织化学 SABC法原位检测肝脏中的HSP70的蛋白表达水平。结果 :2 0 .0 mg· kg-1 硝酸镧组大鼠肝脏中 HSP70阳性细胞数与对照组相比明显增多 ( P<0 .0 5 )。结论 :硝酸镧 ( 2 0 .0 mg· kg-1 )对肝脏损伤时 ,一定剂量Objective:To observe the expression of heat shock proteins 70 (HSP70) in rat livers after long term administration of lanthanum nitrate La(NO 3) 3. Methods:Young Wistar rats were divided into six groups,which were given La(NO 3) 3 at 0.1,0.2,2.0,10.0 and 20.0 mg·kg -1 , respectively, and the control group with physiological saline for six months.The expression of HSP70 was detected in rat livers by immunocytochemistry. Results:The positive cells of HSP70 in the group of 20.0 mg·kg -1 La(NO 3) 3 were much more than those in the control. Conclusion:HPS70 is involved in the anti injury mechanism of rat livers when rat livers were injured at 20.0 mg·kg -1 La(NO 3) 3.

关 键 词：稀土元素 热休克蛋白70 大鼠 WISTAR 肝脏损伤 免疫组织化学 硝酸镧 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]