甘氨酸-钼锗酸盐的体外抑瘤效应  

Inhibitory Effect of (glyH)_3GeMo_(12)O_(40)·3H_2O on Tumor Growth in vitro

作　　者：杨国天[1] 常雅萍[1] 陈强 韩正波[3] 王恩波[3] 

机构地区：[1]吉林大学基础医学院免疫学教研室,吉林长春130021 [2]内蒙古阿尔山林业局医院 [3]东北师范大学化学学院多酸研究所

出　　处：《吉林大学学报（医学版）》2003年第6期773-776,共4页Journal of Jilin University：Medicine Edition

摘　　要：目的 :观察甘氨酸 -钼锗酸盐体外抑瘤效应及毒性作用。方法 :采用氮蓝四唑 ( MTT)掺入法检测甘氨酸 -钼锗酸盐 ( 6.2 5～ 2 0 0 mg· L-1 )对 H740 2、B1 6、BGC- 82 3和 MGC- 80 3肿瘤细胞株增殖的影响 ,对 FL、L92 9正常细胞株生长的影响及对小鼠脾细胞增殖的影响。结果 :一定浓度的甘氨酸 -钼锗酸盐对 H740 2、B1 6、BGC- 82 3和 MGC- 80 3肿瘤细胞株有显著的抑瘤效应 ( P<0 .0 5 ) ;对FL及 L92 9细胞株无毒性作用 ;对小鼠脾细胞有显著的促增殖作用 ( P<0 .0 5 )。结论 :甘氨酸Objective:To investigate the inhibitory effect of (glyH) 3GeMo 12 O 40 ·3H 2O on tumor cell lines and the toxicity effect on normal cell lines in vitro . Methods: The effects of (glyH) 3GeMo 12 O 40 ·3H 2O on proliferation of H7402,B16, BGC 823,and MGC 803 tumor cell lines,its influence on the growth FL, L929 cell lines, and mice splenocytes were determined by MTT method. Results:(GlyH) 3GeMo 12 O 40 ·3H 2O (6.25~200 mg·L -1 ) inhibited the growth of H7402,B16,BGC 823, and MGC 803 tumor cell lines significantly( P <0.05); there was no toxicity on FL and L929 cell lines( P <0.05);it enhanced ConA induced mitogenetic response of splenocytes. Conclusion: (GlyH) 3GeMo 12 O 40 ·3H 2O shows a inhibitory effect on the growth of cancer.

关 键 词：甘氨酸-钼锗酸盐 药物筛选试验 抗肿瘤 脾 免疫学 比色法 氮蓝四唑 

分 类 号：R730.5[医药卫生—肿瘤]