丙戊酸钠对大鼠脑NMDA受体在爪蟾卵母细胞表达的影响  

Effect of Sodium Valproate on the Expression of N-methyl-D-Aspartate Receptor of Rat Brain Tissue in Xenopus Oocytes

作　　者：顾饶胜[1] 陈厚昌[2] 

机构地区：[1]第四军医大学吉林军医学院药理学教研室,吉林吉林132013 [2]第一军医大学药理学教研室

出　　处：《吉林大学学报（医学版）》2003年第6期733-735,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (395 70 82 7)

摘　　要：目的 :观察丙戊酸钠对大鼠脑 N-甲基 - D-天冬氨酸 ( NMDA)受体在非洲爪蟾卵母细胞表达的影响。方法 :采用电压钳位技术记录表达于非洲爪蟾卵母细胞膜上的大鼠脑 NMDA受体通道电流及特性并观察 0 .1和 0 .5 mmol· L-1 丙戊酸钠对大鼠脑 NMDA受体通道电流的影响。结果 :丙戊酸钠 ( 0 .1和 0 .5 mmol· L-1 )对表达于非洲爪蟾卵母细胞的大鼠脑 NMDA受体功能有抑制作用 ,电流峰值由 ( 97.5± 9.8) n A降至 ( 79.7± 1 0 .5 ) n A、( 61 .3± 1 2 .5 ) n A ( P<0 .0 5和P<0 .0 1 )。结论 :丙戊酸钠对大鼠脑Objective:To observe the effect of valproate sodium on the expression of N methyl D aspartate(NMDA)receptor of rat brain tissue in xenopus. Methods:The currents of expressed receptors of NMDA in Xenopus oocytes were detected with the double electrode voltage clamps technique, and the effects of valproate sodium of 0.1,0.5 mmol·L -1 on them were obseved.Results:Valproate sodium inhibited markedly the current of NMDA receptor. The currents decreased from (97.5±9.8)nA to (79.7± 10.5) and (61.3±12.5)nA ( P <0.05 and P <0.01).Conclusion:Valproate sodium inhibites markedly the functions of NMDA receptor of Wistar rats brain.

关 键 词：丙戊酸钠 受体 N-甲基-D-天冬氨酸 大鼠 WISTAR 卵母细胞 爪蟾属 脑 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]