谷氨酸和白细胞介素-1β致痫大鼠海马cAMP含量的变化  

Cyclic AMP Levels in the Hippocampus of Epilepsy Rats Induced by L-glutamate and Interleukin-1β

作　　者：李莉[1] 李正莉[1] 朱长庚[1] 刘庆莹[1] 于珊[1] 李平[1] 

机构地区：[1]华中科技大学同济医学院基础医学院解剖学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2003年第6期582-584,588,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No 3 0 170 484) ;湖北省自然科学基金资助项目 (No 2 0 0 1ABB14 2 )

摘　　要：目的　研究谷氨酸和白细胞介素 1β (interleukin 1β ,IL 1β)致痫大鼠海马组织cAMP含量的变化。 方法 40只SD雄性大鼠随机分为 4组 ,均行侧脑室注射 ,A组注射致痫剂量的L 谷氨酸钠 ;B组联合注射IL 1β和阈下剂量的L 谷氨酸钠 ;C组注射白细胞介素 1受体拮抗剂 (IL 1receptorantagonist ,IL 1ra) 3 0min后 ,再联合注射IL 1β和阈下剂量的L 谷氨酸钠 ;D组注射等容量的生理盐水。各组均在注射完毕 3 0min后断头取海马组织 ,采用放射免疫分析法(RIA)测定 4组大鼠海马的cAMP含量。结果　A、B两组大鼠均有明显的痫性发作 ,且海马cAMP的含量均明显高于D组 (P <0 0 5) ,C组大鼠海马cAMP的含量较B组下降 ,并且与D组无显著差异。结论　在谷氨酸和IL 1β的协同致痫活动中 。Objective To investigate the changes of cyclic adenosine monophosphate (cAMP) levels in th e hippocampus of epilepsy rats induced by L-glutamate and interleukin-1β (IL-1β).Methods Forty adult SD rats were randomly divided into 4 group s (n=10 in each group). Group A was injected with L-glutamate intracere broventricularly (i.c.v.); Group B was injected with IL-1β and L-glutamat e serially in the same way; Group C was injected with IL-1 receptor antagonist (IL-1ra) 30 min followed by injection of IL-1β and L-glutamate; Group D was injected with physiological saline as control group. The animals were sacrif iced 30 min after surgery. The levels of cAMP in the hippocampus homogenate of e ach group were assayed by means of radioimmuoassay (RIA). Results The groups A and B showed epileptic activities. The cAM P levels in group A \[(593 27±172 44) pmol/g\] and group B \[(530 98±128 5 1) pmol/g\] were much higher than those in group D \[(381 19±81 28) pmol/g\] (P<0 05), while the cAMP levels in group C \[(451 95±65 98) pmol/g\] wer e decreased as compared with those in group B, and had no difference with those in group D (P>0 05). Conclusion cAMP may play a role in the onset and continuance of epileptic activities induced by IL-1β and L-glutamate.

关 键 词：谷氨酸 白细胞介素-1Β 海马 CAMP 含量 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]