微胶囊对大鼠糖尿病异种胰岛组织移植免疫隔离作用的实验研究  

Experimental Study of Immunoisolated Effectiveness of Microencapsulating on Xentransplantion of Islet Tissues in Diabetic Rats

作　　者：刘曾旭[1] 刘德明[1] 刘德伍[2] 

机构地区：[1]江西医学院人体解剖学教研室,江西南昌330006 [2]江西医学院第一附属医院烧伤中心,江西南昌330006

出　　处：《江西医学院学报》2003年第6期20-22,共3页Acta Academiae Medicinae Jiangxi

基　　金：江西省科委资助题E960 10 1

摘　　要：目的　观察微胶囊 (海藻酸钠 多聚赖氨酸 海藻酸钠 )对大鼠糖尿病异种胰岛组织移植的免疫隔离作用。方法　糖尿病大鼠 30只。随机分为 5组 :( 1)微囊组 ;( 2 )未包囊组 ;( 3)空囊移植组 ;( 4 )生理盐水组 ;( 5 )糖尿病组。每组各 6只。微囊组用微囊化的小鼠胰岛细胞注入糖尿病大鼠腹腔内。未包囊组用纯化后的胰岛细胞注入糖尿病大鼠腹腔内。空囊移植组用与微囊组数量相同的空微囊注入糖尿病大鼠腹腔内。生理盐水组用等量的生理盐水注入糖尿病大鼠的腹腔内。糖尿病组对糖尿病大鼠不行移植操作。各组大鼠分别于移植后 2h、4h测量血糖 ,以后每天测定血糖 1次 ,1周后改为每 10d测 1次 ;同时称量体重 ,观察饮水量及尿量 ,直至空腹血糖高于 19.3mmol/l定为胰岛移植物排斥。结果　糖尿病组、空囊移植组及生理盐水组大鼠血糖无明显变化 ;微囊组糖尿病大鼠异种胰岛移植 2h后血糖明显低于未包裹组 ,下降幅度为 8～ 12mmol /L ,有显著性差异 (P <0 .0 1) ;未包裹组血糖下降仅维持 2～ 3d ,微胶囊组血糖下降可持续 5 6～ 12 0d ,两组持续天数比较 ,有显著性差异 (P <0 .0 1)。移植后 1个月从腹腔回收的微囊胰岛呈圆型、表面光滑 ,无明显纤维组织包绕。囊内胰岛DTZ染色呈桔黄色。结论　微胶囊制作技术可明显延长小?Objective To test immunoislated effectiveness of microencapsulated mice islets as xenografts in diabetic rats.Methods The diabetic rats were divided into five groups at random, each of which had six rats, including①microencapsulated group;②unencapsulated group;③empty capsules group;④physiological saline solution group;⑤diabetes group.Microencapsulated mice islet cells,the pure mice islet cells, the equivalence of empty capsules,and the equivalence of physiological saline solution were injected into peritoneal cavity of diabetic rats of microencapsulated group,of unencapsulated islet group,of empty capsules group,and of physiological saline solution group,respectively.In diabetic group,the diabetic rats did not perform operation.The blood sugar of all diabetic rats was measured at two hours and six hours after transplants,then measured once every day for a week followed by once every ten days thereafter.In the meantime,we weighed the rats,measured the quantity of the rats' drink and urine.While the limosis blood sugar of diabetes rats was above 19.3 mmol/L,we considered that immunity repulsion to the xenografts was happened. Results Intraperitoneal xenotransplantion of microencapulated mice islets into rats peritoneal cavity with the STZ induced diabetic state was reversed apparently at two hours after islets transplantation and normoglycemia was maintained for up to 56～120 days.The depressive breadth was 8～12 mmol/L( P <0.01).The diabetic rats receiving transplants of unencapsulated islets experienced normoglycemia for 2～3 days.In contrast,the diabetic states were maintained in control groups of receiving empty capsules or 0.9%Nacl solution and diabetes group.The retrieved microcapsules in the 30th day posttransplantion were perfectly intact,spherical,smooth and poorly fibroblast on the capsules surface.The islets in the capsules were stained orange yellow with DTZ.Conclusion These results demonstrated that microencapsulated preparation technique could prolong survival of mice pancreatic

关 键 词：微胶囊 糖尿病 胰岛移植 免疫 隔离 动物 实验 大鼠 

分 类 号：R587.1[医药卫生—内分泌]