构建重组肿瘤基因疫苗对肿瘤进展及生存率影响的实验结局(英)  

Effect of recombinant genetic vaccine against tumors on tumor progression and survival rate of mice

作　　者：倪兵 吴玉章 李艳秋 唐艳 邹丽云 周伟 贾正才 林治华 

机构地区：[1]解放军免疫学研究所,重庆市400038

出　　处：《中国临床康复》2003年第32期4325-4327,共3页Chinese Journal of Clinical Rehabilitation

基　　金：973国家计划资助项目(2001CB510001)~~

摘　　要：目的:利用P815肿瘤动物模型观察IL-12对肿瘤动物模型抗肿瘤作用的效应。方法:将小鼠肥大细胞瘤P815特异抗原基因P1A克隆到真核表达质粒pCI-neo中;用P815细胞对DBA/2小鼠右腹侧皮下注射,构建P815小鼠肿瘤模型;以重组基因疫苗单独或与鼠IL-12真核表达质粒一起肌肉注射,观察肿瘤的消长、特异细胞毒T淋巴细胞激活和抗体的生成情况。结果:重组基因疫苗在体外有很好的表达,注射后细胞毒性T淋巴细胞(CTL)的杀伤效率为40%,IL-12共注射的CTL杀伤效率达到60%。免疫后,30%小鼠的肿瘤出现消退;同IL-12共注射则有50%的小鼠的肿瘤出现消退。两种情况下都不能检测到任何特异抗体的产生。结论:长期存在的IL-12可以持续刺激机体细胞免疫,使肿瘤的生存环境持续恶化,从而达到治疗肿瘤的目的。AIM:To study the immunological effect of genetic vaccine co administered with murine IL 12 against mouse mastocytoma P815. METHODS:Antigen specific P1A gene of P815 mastocytoma was transduced into the eukaryotic expression vector pCI neo to prepare the genetic vaccine.Mouse models of P815 tumor were established by subcutaneous injection of P815 cells,and the growth of the tumor, the stimulation of cytotoxic T cells and production of specific antibodies were observed following injection of the recombinant vaccine into mice muscle alone or together with IL 12 expression plasmids. RESULTS:The recombinant vaccine could be expressed in vitro, with specific release rate of cytotoxic T cells reaching 40%after injection alone, which mounted up to 60%when IL 12 expressing plasmid was co administered.About 30%of the recombinant P1A vaccine inoculated mice showed signs of tumor regression, and when IL 12 expressing plasmid was used,the regression could reached 50%.No antibodies, however, could be detected in the sera of any of the immunized mice. CONCLUSION:Tumor specific immunological response can be elicited by recombinant P1A vaccine through cellular immunity,and IL 12 can enhance the immune effect of this vaccine.

关 键 词：肿瘤基因 疫苗 生存率 淋巴细胞 抗体 

分 类 号：R730[医药卫生—肿瘤]