前列腺癌染色体8p^(22)-8p^(12)基因杂合性丢失研究  

A STUDY ON ALLELIC LOSS OF HETEROZYGOSITY OF CHROMOSOME 8p^(22)-p^(12) IN CHINESE PROSTATE CANCER

作　　者：毛庆波[1] 仇成轩[1] 刘虎[1] 

机构地区：[1]济宁医学院病理学教研室

出　　处：《济宁医学院学报》2003年第4期1-3,共3页Journal of Jining Medical University

基　　金：山东省卫生厅青年科学基金资助项目 ( 99CA2CEA2 )

摘　　要：目的　探讨中国人前列腺癌染色体 8p2 2 ～ 8p12 基因杂合性丢失的发生率及细胞生物的意义。方法　采用PCR—变性聚丙烯酰胺凝胶电泳和硝酸银染色技术 ,对 32例前列腺癌组织及癌旁组织进行 8p2 2- 8p12 区域内的 5个微卫星多态标志物等位基因杂合性丢失 (LOH)分析。结果　 5个微卫星平均发生率为76 .6 % ,其中LPL - 3GT位点LOH发生率最高 (88 9% ) ,D8S87位点最低 (5 9 1 % ) ;8p2 2 - p12 位点LOH发生率与肿瘤分化密切相关。其中D8S133和D8S135位点LOH发生率与肿瘤分化程度呈正相关 (P <0 0 1 ) ,LPL - 3GT、NEFL和D8S87位点LOH与肿瘤细胞分化程度无统计学意义 (P >0 0 5 )。结论　研究结果提示 ,前列腺癌 p2 2 - 8p12 是LOH的频发区域 ,该区域等位基因LOH与前列腺癌的发生密切相关。不同位点LOH的发生其细胞生物学意义不同 ,提示Objective The aims of the study were to investigate the frequency and their cellular biological implicates of loss of heterozygosity (LOH) on chromosome 8p 22 -p 12 in Chinese prostate cancers.Methods The polymerise chain reaction(PCR) denaturing polyanylamid and gels staining with silver salts techniques were used,and the LOH status on chromosome 8p 22 -p 12 in 32 Chinese prostate cancer tissues were studies with 5 microsatellite markers Results The average rate of the average rate of LOH on chromosome 8p 22 -p 12 in 5 loci was 76 6% The highest frequency LOH occurred at LPL-3GT locus (88 9%) on chromosome 8p 22 and the lowest occurred at D8S 87 (59 1%) on chromosome 8p 12 The high frequency of LOH at D8S 131 and D8S 135 loci were statistically associated with the high grade cancers (grade Ⅲ vs grade I,P<0 01) The study suggested that the LOH rates on chromosome 8p 22 -p 12 were relatively high in Chinese prostate cancers and they may be related closely to the development (genesis and progression) of prostate cancer Conclusion Some tumor suppressor genes of having different functions might existence on chromosome 8p 22 -p 12 Further studies were needed to identity the possible specific genes of prostate cancer

关 键 词：前列腺癌 染色体 8p^22—8p^12 基因杂合性丢失 

分 类 号：R737.25[医药卫生—肿瘤]