单胺氧化酶B基因微卫星多态与帕金森病的相关分析  

Correlated analysis on the monoamine oxidase B gene GT repeat polymorphism and the Parkinson's disease

作　　者：郝怡鑫[1] 吴奇涵[2] 谢惠君[3] 徐玲[2] 汤国梅[2] 汪晓华[2] 陈丽珊[2] 任大明[2] 

机构地区：[1]第二军医大学附属长海医院实验诊断科,上海200433 [2]复旦大学遗传学研究所国家重点遗传工程实验室 [3]第二军医大学附属长海医院神经内科

出　　处：《中华神经科杂志》2003年第6期418-421,共4页Chinese Journal of Neurology

摘　　要：目的　研究上海地区汉族人群中单胺氧化酶B(MAOB)基因第二内含子中鸟嘌呤、胸腺嘧啶 (GT)二碱基重复的微卫星多态位点与帕金森病 (Parkinson’sdisease ,PD)易患性之间的关系。方法　采用扩增片段长度多态法 (Amp FLP) ,在上海汉族人群中选择 6 7例散发PD患者为PD组和2 0 4名健康者为对照组 ,运用微卫星荧光标记 半自动基因分型技术精确计算出微卫星等位基因片段大小 ,进而分析该多态在PD易患性中的作用。结果　该位点短片段 (≤ 170bp)等位基因的分布在PD组中明显高于与对照组 (χ2 =11 2 8,P =0 0 0 1) ,而 172bp等位基因在PD组中亦明显低于与对照组 (χ2 =5 16 ,P =0 0 2 3)。结论　MAOB基因GT二碱基重复多态位点与本组PD患者的易患性有关。Objective To investigate the relationship of the GT dinucleotide repeat polymorphism in intron 2 of the monoamine oxidaseB gene (MAOB,Xp15.21-4) and Parkinson’s disease(PD).Methods The samples are recruited from Shanghai Han population,including 67 sporadic PD patients and 204 health controls.Using Amp-FLP and microsatellite fluorescence-based semiautomatic genotype analysis technique,the DNA fragment length of microsatellite polymorphism was precisely calculated.Furthermore,the role of the microsatellite in sensitivity to PD was observed. Results With χ 2 test,the (GT)n repeat alleles being shorter than or equal to 170 base pairs in the intron 2 of the MAOB gene were found significantly associated with PD(χ 2=11.28, P =0.001).The distribution of 172 base pair allele in PD cases was significantly lower than those controls(χ 2=5.16, P =0.023).Conclusion (GT)n repeat polymorphism in MAOB gene is probably associated with PD’s onset.

关 键 词：单胺氧化酶B 基因多态性 帕金森病 易患性 扩增片段长度多态法 基因分型 

分 类 号：R742.5[医药卫生—神经病学与精神病学] Q55[医药卫生—临床医学]