TNF-αmRNA、TNF-R1mRNA在慢性肝病肝组织中的表达及意义  

Significance of TNF-αmRNA and TNF-R1mRNA Expressing in Chronic Liver Disease

作　　者：陈昊[1] 白玲[1] 张昶[1] 杨聪颖[1] 王巧[1] 

机构地区：[1]江苏省连云港市第一人民医院病理科,222002

出　　处：《齐齐哈尔医学院学报》2003年第12期1321-1322,共2页Journal of Qiqihar Medical University

摘　　要：目的　探讨TNF -α和TNF -R在慢性肝炎、肝硬化及肝癌发生、发展中的调节作用。方法　原位分子杂交法检测了 94例慢性肝病肝组织中TNF -αmRNA、TNF -R1mRNA的表达 ,其中慢性肝炎 2 8例、肝硬化 2 2例、肝癌 4 4例。结果　慢性肝炎、肝硬化、肝细胞肝癌组织中TNF -αmRNA阳性率分别为 5 3.6 %、86 .4 %、4 3.2 % ;TNF -R1mRNA阳性率分别为 4 6 .4 %、90 .9%、38.6 %。两者同时阳性分别为慢性肝炎 7例 (2 5 % )、肝硬化 18例 (81.81% )、肝细胞肝癌 11例 (2 5 % ) ;两者同时阴性分别为 9例 (32 .14 % )、1例 (0 .0 5 % )、19例 (4 3.19% )。慢性肝炎与肝硬化TNF -αmRNA阳性率相比 ,P<0 .0 5 ,慢性肝炎与肝硬化TNF -R1mRNA阳性率相比 ,P <0 .0 1,差异有高度显著性 ;肝硬化与肝癌TNF -αmRNA和TNF -R1mRNA阳性率相比 ,P <0 .0 1,差异有高度显著性 ;慢性肝炎与肝癌TNF -αmRNA和TNF -R1mRNA阳性率相比 ,P >0 .0 5 ,差异无显著性。慢性肝炎的活动度越重其表达率越高 ,TNF -αmRNA和TNF -R1mRNA表达强度也强 ,肝硬化组的阳性强度最强 ,肝细胞肝癌组织表达与分化程度关系不密切。结论　TNF -α和TNF -R在肝硬化的发生、发展中起重要作用 ,对慢性肝炎亦有一定的作用 ;肝细胞肝癌时TNF -αmRNA和TNFObjective To study the role of TNF-αand TNF-R in the chronic liver disease . Methods The in situ hybridization (ISH) method was used to examine the expression in the hepotocyter of 94 cases of the chronic liver disease , which included 28 cases chronic hepatitis ,22 cases liver cirrhosis and 44 cases hepatocellular carcinoma . Results In the chronic hepatitis group,chronic hepatitis group and hepatocellular carcinoma group , the positive rates of TNF-αmRNA were 53.6%, 86.4%, 43.2% respectively , and in that the positive rates of TNF-R1mRNA were 46.4%,90.9%,38.6% respectively. The expression of TNF-αmRNA and TNF-R1mRNA in liver cirrhosis group was more evident than chronic hepatitis group and hepatocellular carcinoma group respectively (P< 0.01). There was no statistic difference between the chronic hepatitis group and the hepatocellular carcinoma group (P>0.05). Conclusions TNF-αand TNF-R play an important role in the course of liver cirrhosis ,and is involved in the course of chronic hepatitis either . The expression of TNF-αmRNA and TNF-R1mRNA in hepatocellular carcinoma is greatly decreased than that in liver cirrhosis.

关 键 词：TNF-Α TNF-R1 慢性肝病 肝组织 临床意义 原位分子杂交法 肝硬化 

分 类 号：R575[医药卫生—消化系统]