Content　of　Androgen　Receptor　in　Cultured　Genital　Skin　Fibroblast　From　Different　Ages　of　Chinese　Normal　Men  

作　　者：卢建 何立敏 张金山 杨震 周云 

机构地区：[1]DepartmentofPathophysiology,SecondMilitaryMedicalUniversity,Shanghai200433 [2]DepartmentofSurgery,ShuzhouChildren＇sHospitalShuzhou,215003

出　　处：《Journal of Reproduction and Contraception》1995年第1期14-18,共5页生殖与避孕（英文版）

摘　　要：A rapid, simple,reliable method is described for assaying androgen receptor (AR) in dispersed, whole,cultured human genital skin fibroblasts (GSF) with a synthetic androgen, H-methyltrienolone (3H- R1881). Receptors for androgen in GSF exhibit high affinity (Kd=3.01 0.1 nmol/L), low binding capacity and androgen sped/icity.The content of AR in cultured GSF from 40 normal men varying in age from 1.5-60 years was also investigated by this assay.Scatchard analysis and single plot revealed the presence of 4500-8500 binding sites per cell, mean number of AR in GSF of these men is 628811082 binding sites/cell.NO signijicant difference was observed in the content of AR in different age groups. This result showed that the content of AR in these cells did not change with age.A rapid, simple,reliable method is described for assaying androgen receptor (AR) in dispersed, whole,cultured human genital skin fibroblasts (GSF) with a synthetic androgen, H-methyltrienolone (3H- R1881). Receptors for androgen in GSF exhibit high affinity (Kd=3.01 0.1 nmol/L), low binding capacity and androgen sped/icity.The content of AR in cultured GSF from 40 normal men varying in age from 1.5-60 years was also investigated by this assay.Scatchard analysis and single plot revealed the presence of 4500-8500 binding sites per cell, mean number of AR in GSF of these men is 628811082 binding sites/cell.NO signijicant difference was observed in the content of AR in different age groups. This result showed that the content of AR in these cells did not change with age.

关 键 词：Androgen receptor Foreskin FIBROBLAST 

分 类 号：R339.21[医药卫生—人体生理学]