掺锰纳米PbO_2修饰电极色谱电化学用于四氢生物喋呤等物质的检测研究  

Determination of tetrahydrobiopterin and other related compounds by Mn-doped PbO2 modified electrode

作　　者：万芳利[1] 张文[1] 顾静[1] 张贵荣[1] 谢云峰[1] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《化学传感器》2003年第4期15-22,共8页Chemical Sensors

基　　金：高新技术发展计划(No.2002AA601330);上海市科委(No.021409012)资助项目

摘　　要：该文报道了一种新型掺锰纳米二氧化铅修饰电极,将该修饰电极用于生物体内重要的辅酶四氢生物喋呤(tetrahydrobipterin,BH_4)、单胺类神经递质多巴胺(DA)及其代谢产物的检测,具有很高的灵敏度和稳定性。将此电极作为液相色谱电化学检测器与微渗析取样技术联用,成功检测了外源性的BH_4对大鼠纹状体中多巴胺及其代谢产物浓度的影响。A novel nanocrystalline Mn - doped lead dioxide deposited film modified glassy carbon electrode was prepared. The novel modified electrode showed favorable electrocatalytic behavior towards dopamine and its metabolites with high sensitivity and stability. We took this electrode as electrochemistry detector, cooperating with high performance liquid chromatography and microdialysis, to detect how lelrahydrobipterin(BH4) effects the concentration of dopamine and its metabolites in rat striatum. It has been shown that the external BH4 does effect the content of BH4 and dopamine and its metabolites in striatum.

关 键 词：掺锰纳米二氧化铅修饰电极 色谱电化学 四氢生物喋呤 多巴胺 微渗析 检测 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]