热带假丝酵母对癸烯-1及十二烯-1的两端氧化  

DITERMINAL OXIDATION OF DECENE-1 AND DODECENE-1 BY CANDIDA TROPICALIS

作　　者：刘祖同[1] 易祖华[2] 

机构地区：[1]清华大学生物科学与技术系,北京100084 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1992年第5期340-345,共6页Acta Microbiologica Sinica

摘　　要：研究了热带假丝酵母突变株 SD_6分别对癸烯-1及十二烯-1的两端氧化。在癸烯-1的主要代谢物中有癸二酸、壬二酸和辛二酸,而在十二烯-1的主要代谢物中含十二碳二元酸、十一碳二元酸、癸二酸和壬二酸。这些产物均通过 GC 和 GC-MS 分析确定。根据这些结果提出了烯烃-1的微生物两端氧化途径。从烯烃-1得到同碳链的及比其少一个碳原子的饱和二元酸表明,在该菌的烯烃-1代谢中,除末端甲基的氧化外,另一末端双键的水合作用可能起着重要作用。The diterminal oxidation of decene-1 and dodecene-1 by a mutant SD_6 derived from Can-dida tropicalis was studied.The main metabolites from decene-1 comprised suberic,azelaic andsebacic acid,and those from dodecene-1 consisted of azelaic,sebacic,undecanedioic and dode-canedioic acid.These products were identified by GC and GC-MS analyses.Based on the resu-lts,the microbial metabolic pathways of alkene-1 were proposed.In the course of alkene-1 (?)ter-minal oxidation in the strain,the hydration of the terminal double bond of alkene molecule mayplay an important role,except oxidation of the saturated termination.

关 键 词：热带假丝酵母 烯烃 两端氧化 

分 类 号：Q939.506[生物学—微生物学]