c-myc基因表达与白血病细胞分化诱导及增殖受抑的相关性  

Relationship Between Different c-myc Expression in Differentiation or Proliferative Suppression of HL-60 Cells

作　　者：袁跃传[1] 沈素芸[1] 

机构地区：[1]第四军医大学西京医院血液科,西安710032

出　　处：《中国肿瘤生物治疗杂志》1996年第1期48-50,共3页Chinese Journal of Cancer Biotherapy

摘　　要：采用0.5％～1.0％低血清培养法较好地模拟了小剂量rhTNF-α(50U/ml)对人早幼粒白血病细胞株HL-60的增殖抑制效应,但未发现促分化作用,对细胞株c-myc基因的表达亦无明显影响;而TNF(50μ/ml)具有诱导HL-60细胞分化和抑制细胞增殖的作用,且显著抑制其c-myc癌基因的表达水平。结果表明c-myc基因表达减低主要与TNF诱导HL-60白血病细胞分化相关,并非细胞增殖受抑的结果,对癌基因表达水平的调控可能是小剂量TNF发挥分化诱导作用的重要机制。We compared the changes of c-myc expression, cell growth kinetics, ability to reduce NBT and 3H -TdR incorporation rate of HL-60 cells in low concentration of FCS, or treated by TNF or no treatment, respectively. The results showed that, although the prolifcrative suppression of HL-60 cells treated by 50U/ml TNF was mimicked by adding 1. 0%and 0. 5%FCS in culture media, the differentiation had notappeared and it had not effected on c - myc expression, either. TNF (50U/ml) could inhibit proliferation of HL-60 cells during inducing differentiation and the expression of c-myc oncogene decreased remarkedly. The results indicated that decreased expression of c-myc in TNF treated HL-60 cells were mainly connected with differentiate maturation but not with proliferative suppression.

关 键 词：C-MYC基因 表达 白血病 细胞分化 诱导 增殖受抑 相关性 

分 类 号：R733.7[医药卫生—肿瘤]