癌相关抗原粘蛋白前体诱导人肿瘤引流区淋巴结的细胞毒作用  

Cytotoxicity of Human Tumor Draining Lymph Node Cells Stimulated by Cancer-Associated Apomucin in vitro

作　　者：马运国[1] 袁玫[1] 李力[1] 费丽华[1] 

机构地区：[1]解放军总医院肿瘤生物学研究室,北京100853

出　　处：《中国肿瘤生物治疗杂志》1996年第3期183-185,共3页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学资金(395707792)

摘　　要：人肿瘤相关抗原可诱导宿主的细胞免疫反应已被证实。一些肿瘤粘蛋白也证实可诱发T细胞细胞毒作用。本研究是观察从胰腺癌细胞株提取的粘蛋白核芯肽诱导人肿瘤引流区淋巴结细胞的抗肿瘤免疫作用。4例乳腺癌、3例胃癌、3例结肠癌的肿瘤引流区淋巴结手术切除后立即在体外培养于含50μg/ml胰癌核芯肽及50单位IL-2的培养基中,观察其对多种癌细胞的杀伤效应(MMT法)。结果表明体外经胰癌核芯肽刺激后增殖的淋巴结细胞对多种肿瘤细胞有杀伤作用,如结肠癌、胃癌、胰腺癌及白血病细胞K562。本研究结果为以肿瘤粘蛋白核芯肽制备疫苗用于主动特异性免疫治疗或过继免疫治疗打下基础。Human tumor antigen elicit a cell-mediate immune response in the host have been identified. In this study, a pancreatic cancer cell line SW1990 associated apomucin was examined for the induction of tumor draining lymph node cells(TDLN) anti-tumor immune responses.The TDLN of patients with breast cancer(4 cases), gastric cancer(3 cases), colon cancer(3 cases) were isolated, cocultured with apomucin 50 μg/ml and low dose rhIL-2 50U/ml in vitro, the cytolytic effects were measured by MTT assay. The results showed that: the proliferated lymphocytes can lysis LS174T colon cancer cells, KATO-Ⅲ gastric cancer cells, SW1990 pancreatic cancer cells and K562 leukemia cells. The most cytolytic effects were higher than LAK. This results might provide a rational basis for future attempts to elicit and manipulate apomucin vaccine for active specific immunotherapy or adoptive immunotherapy for adenocarcinoma.

关 键 词：癌相关抗原 粘蛋白前体 诱导 肿瘤引流区 淋巴结 细胞毒作用 

分 类 号：R730.3[医药卫生—肿瘤]