腹腔内注射IL-2重组腺病毒、IL-3重组腺病毒增强化疗药物抗白血病作用的研究  

Enhancing Effects of Intraperitoneal Injection with IL-2 Recombinant Adenovirus and IL-3 Recombinant Adenovirus on the Anti-Leukemia Activity of Chemotherapy

作　　者：于敏[1] 杨建民[1] 孟沛霖[1] 曹雪涛[2] 章卫平[2] 

机构地区：[1]第二军医大学附属长海医院血液科,上海200433 [2]第二军医大学免疫学教研室,上海200433

出　　处：《中国肿瘤生物治疗杂志》1996年第3期191-194,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金;优秀中青年人才专项基金(39421009)

摘　　要：对实验性白血病小鼠模型大剂量化疗后,直接腹腔注射IL-2重组腺病毒(Ad-IL-2)和/或IL-3重组腺病毒(Ad-IL-3),发现腹腔注射对照腺病毒载体小鼠虽经大剂量化疗,仍有大量白血病细胞浸润至骨髓、血管、肝脏及脾脏。而腹腔注射Ad-IL-2或Ad-IL-3组小鼠肿瘤生长缓慢,注射Ad-IL-2组小鼠脾NK、CTL活性显著提高,注射Ad-IL-3组小鼠腹腔巨噬细胞数量及杀伤活性明显提高,联合应用Ad-IL-2,Ad-IL-3组小鼠抗白血病作用最为明显,骨髓中虽然仍见白血病细胞,但可见较多正常造血细胞,且肝、脾中未见白血病细胞浸润。表明腹腔内注射Ad-IL-2和Ad-IL-3可显著增强大剂量化疗对白血病的治疗效果。The Ad-IL-2 and/or Ad-IL-3 were i. p. injected into experimental leukemic mice after high dose chemotherapy. When Ad - LacZ was i. p. injected into leukemic mice, there were leukemic cells in bone marrow, vessal, liver and spleen. After i. p. injection of Ad-IL-2 and/or Ad-IL-3, the leukemia - bearing mice showed significant inhibition of tumor growth. As i.p. injection of Ad-IL-2, the splenic NK, CTL activity increased; as i. p. injection of Ad - IL - 3, the number and cytotoxicity of peritoneal macrophages were significantly higher than that of the control groups. After i. p. injection of Ad - IL - 2 and Ad - IL - 3 at the same time, the anti - leukemia activity was the best. Though there were less leukemic cells in the bone marrow, there were no leukemic cells in the liver and spleen. Our results suggested that i. p. injection of Ad - IL - 2 and Ad - IL - 3 could significantly enhance the anti - leukemia activity of chemotherapy.

关 键 词：腹腔内注射 IL-2 重组腺病毒 IL-3 重组腺病毒 抗白血病作用 化疗药物 

分 类 号：R733.7[医药卫生—肿瘤]