肿瘤坏死因子α和干扰素α与丝裂霉素C在体外的协同细胞毒作用  

Synergistic Enhancement of Cytotoxicity of Mitomycin C on Gastric Cancer Cells in vitro by Tumor Necrosis Factor a and Interferon α

作　　者：田凤岐[1] 张学庸[1] 樊代明[1] 牟震先[1] 吴觉平[1] 

机构地区：[1]第四军医大学附属西京医院消化内科,西安710033

出　　处：《中国肿瘤生物治疗杂志》1996年第3期195-198,共4页Chinese Journal of Cancer Biotherapy

摘　　要：为了观察肿瘤坏死因子α(TNFα)和干扰素α(IFNα)对丝裂霉素C(MMC)体外杀伤胃癌细胞的影响,利用MTT法检测其对胃癌细胞系和原代胃癌细胞的细胞毒作用。TNFα和/或IFNα(各0～5000U/ml)只对10例细胞中的1例有杀伤力,并与剂量有关,且二者共同作用时杀伤力更强。在3例细胞的观察中,发现TNFα(30U/ml)或IFNα(100U/ml)能分别提高MMC对2例细胞的杀伤力。两种细胞因子联合应用时,可提高MMC对全部10例细胞的杀伤力。经统计学处理,发现TNFα与IFNα、TNFα和/或IFNα与MMC之间有协同作用,结果显示,TNFα和IFNα能协同提高MMC体外细胞毒作用,联合应用可能给临床肿瘤治疗带来益处。The aim is to investigate the effects of tumornecrosis factor α(TNFα0 and interferon a(IFNα) on the cytotoxicity of mitomycin C(MMC) in vitro. The cytotoxicity of the three agents against gastric cancer cell line and freshly isolated gastric cancer cells were determined by the MTT assay. TNFα and/or IFNα (each 0~5000U/ml) showed cytotoxicity effects only on one of ten cell cultures dose-dependently; the combination resulted in a more significant effect. The combination of MMC with TNFα (30U/ml) or IFNα(l00U/ml) could enhance the cytotoxicity of MMC on two of three cell cultures synergisticly. When three agents were used together, the cytotoxicity of MMC against the ten cell cultures was enhanced synergisticly. TNFα and IFNα could enhance the cytotoxicity of MMC synergisticly, and this may make the cancer chemotherapy more effective.

关 键 词：肿瘤坏死因子Α 干扰素Α 丝裂霉素C 体外 协同细胞毒作用 胃癌细胞 

分 类 号：R735.2[医药卫生—肿瘤]