不同剂量rhGM-CSF cDNA直接注射小鼠骨骼肌的表达  

Various Amounts of rhGM - CSF cDNA Transfer Directly into Mice's Muscle in vivo

作　　者：叶棋浓[1] 王恒梁[1] 苏国富[1] 张述[2] 鲍云华 赵瑞华[3] 

机构地区：[1]北京生物工程研究所,北京100071 [2]北京北太平路医院肿瘤一科 [3]中日友好医院

出　　处：《中国肿瘤生物治疗杂志》1996年第3期226-229,共4页Chinese Journal of Cancer Biotherapy

摘　　要：将表达人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)的重组真核表达质粒(pCG1),以30μg和60μg的剂量分别直接注射小鼠骨骼肌,观察不同剂量的质粒(pCG1)在小鼠体内分泌表达rhGM-CSF的情况。ELISA检测表明,注射60μg质粒DNA的小鼠血液中测得的rhGM-CSF含量与对照组相比有显著差别,而注射30μg质粒DNA的小鼠血液中rhGM-CSF含量与对照组相比无显著差别。以60μg质粒DNA直接注射骨骼肌后第15～20天左右表达量最高,平均约91ng/ml。生物学活性检测结果显示,注射60μg质粒DNA的小鼠血液能维持GM-CSF依赖的TF-1细胞的生长。这提示用质粒DNA直接注射小鼠骨骼肌时,质粒DNA必须达到足够的量。We have transferred the different amounts of the plasmid DNA pCGl, which was constructed by cloning rhGM-CSF gene into the eukaryotic expression plasmid pCDS, directly into the BALB/c mice's quadriceps pretreated with bupivacaine. The result of ELISA confirmed that rhGM - CSF was expressed and secreted in mice of group B injected 60μg of plasmid pCGl, but not in mice of group C injected 30μg of plasmid pCGl. The time for the highest level of rhGM-CSF expression was during the fifteenth to twentieth days after plasmid DNA pCGl was injected. Meanwhile, the results of biological activity detection showed that mice's serum of group B (injected 60μg) had the function of retaining the growth of TF - 1 cells. This demonstrates that the rhGM-CSF in mice's serum of group B has certain biological activity. This Model of animal will blaze a new trail in the treatment and prevention of the leukyopenia.

关 键 词：RHGM-CSF CDNA 直接注射 小鼠 骨骼肌 表达 基因治疗 基困转染 

分 类 号：R730.54[医药卫生—肿瘤]