α-肿瘤坏死因子基因修饰人肝癌细胞经^(60)Co辐射后细胞因子的持续分泌  

The Persistent Secretion of TNF-α by60Co Irradiated TNF Gene Modified Human Hepatoma Cell

作　　者：朱敏[1] 徐荣婷[1] 王克敏[1] 厉兴君[1] 胡亮[1] 陈诗书[1] 

机构地区：[1]上海第二医科大学人类基因研究中心,上海200025

出　　处：《中国肿瘤生物治疗杂志》1996年第4期277-279,共3页Chinese Journal of Cancer Biotherapy

基　　金：863’高科技;国家自然科学基金

摘　　要：α-肿瘤坏死因子(α-TNF)具有促肿瘤细胞自溶和增加肿瘤细胞的HLA抗原表达的作用,故将含信号肽的人α-TNFcDNA构建入可表达载体质粒pLXSN,体外包装成缺陷型逆转录病毒后感染人肝癌细胞株(HHCL),经筛选后得到HHCL-TNF细胞株。经不同强度的^(60)Co辐射后,测其24小时的TNF分泌量。观察^(60)Co辐射强度与TNF分泌量的关系及辐射后细胞存活期的差异。将上述辐射后细胞放入液氮冻存,复苏观察TNF分泌量的变化。经研究发现:(1)HHCL-TNF经过^(60)Co辐射后仍能持续分泌约四周,40～100G辐射强度间无显著差异;(2)HHCL-TNF细胞^(60)Co辐射后液氮冻存一段时间,复苏后仍能持续分泌TNF约三周。这些结果为进一步研究应用‘肿瘤疫苗’提供了一定的依据。α-tumor necrosis factor can enhance tumor cells autolysis and the expression of HLA antigenes. So we infected Human Hepatoma Cell Line (HHCL)with recombinant retroviral vector containing an insert of human tumor necrosis factor-α cDNA. The transduced tumor cells were isolated by G418 selection. After being irradiated by different doses of 60Co, their expression of TNF in 24 hours were tested. Observations of the relationship between the doses of irradiation and the expression levels of TNF were done. We also preserved the irradiated HHCL-TNF cells into liquid nitrogen, after recovering, tested their expressions of TNF again. This paper shows that: (l)following irradiation by 60Co, the production of α-TNF persisted for about four weeks, and there is no difference among the different irradiated doses(40G^100G); (2)After being irradiated and kept into liquid nitrogen, the recovering HHCL-TNF cells constitutively secrete α-TNF for about three weeks.These findings provide a logical basis for designing protocols for active immunotherapy in humans.

关 键 词：Α-肿瘤坏死因子 基因修饰 肝癌细胞 ^60CO辐射 细胞因子 持续分泌 

分 类 号：R735.7[医药卫生—肿瘤]