用ELISA和Dot-ELISA方法检测植物病原的比较研究  

THE STUDY OF COMPARISON BETWEEN ELISA AND DOT-ELISA TO DETECT TBB-MLO TSWV AND PSEUDOMON AS SOLAN ACEARUM

作　　者：王远程[1] 蔡少华[1] 谢云陆[2] 何礼远[2] 徐惠迪 

机构地区：[1]中国农业科学院生物技术研究中心,北京100081 [2]中国农业科学院植物保护研究所,北京100094 [3]美国农业部贝尔茨维尔农业研究中心

出　　处：《微生物学通报》1992年第4期237-240,共4页Microbiology China

摘　　要：比较了ELISA和Dot-ELISA方法检测番茄巨芽类菌原体(Tomato Big Bud Mycoplatma-Like Organism,TBB-MLO)、番茄斑萎病毒(Tomato Spotted Wilt Virus,TSWV)和青枯假单胞菌(Pseudomonas solanacearum)的差异,结果表明,两种方法对番茄斑萎病毒无明显差异,对番茄巨芽类菌原体,用Dot-ELISA比用ELISA的检测灵敏度高50—100倍,对青桔假单胞菌,用Dot-ELISA比用ELISA检泓抗原的灵敏度高20倍,而检测抗体的灵敏度高100倍。Tomato big bud mycoplasma-like organism (TBB-MLO) Tomato spotted wilt virus (TSWV) and P. solanacearum was detected by using ELISA and Dot-ELISA methods. The risults showed that to detect TBB-MLO and P. solanacearum Dot-ELISA was about 50—100 times sensitive than ELISA method, but to detect TSWV it gave same results of both methods.

关 键 词：植物病原 检测方法 ELISA 

分 类 号：S432[农业科学—植物病理学]