Neuroprotective effects of nimodipine and MK-801 on acute infectious brain edema induced by injection of pertussis bacilli to neocortex of rats  

作　　者：陈立华 刘丽旭 杨于嘉 刘运生 曹美鸿 

机构地区：[1]Department of Neurosurgery

出　　处：《Chinese Journal of Traumatology》2003年第2期118-123,共6页中华创伤杂志（英文版）

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No .39470 2 33) .

摘　　要：Objective: To explore the mechanism and type of acute infectious brain edema induced by injection of pertussis bacilli (PB) in rat neocortex, to study the neuroprotective effect of non-competitive antagonist of N-methl-D-aspartate ( NMDA ) receptor ( MK-801 ) and antagonist of Ca 2+ channels ( nimodipine )on brain edema, and to investigate the relationship between percentage of water content and cytosolic free calcium concentration ( i) in synaptosomes or content of Evans Blue (EB). Methods: 95 SD rats were randomly divided into five groups, ie, normal control group, sham-operated control group, PB group, nimodipine treatment group and MK-801 pretreatment group. The acute infectious brain edema was induced by injection of PB into the rats. Quantitative measurements of water content and the concentration of EB were performed. i was determined in calcium fluorescent indication Fura-2/AM loaded neuronal synaptosome with a spectrofluorophotometer. To observe the effect of MK-801 and nimodipine, we administered MK-801 48 hours and 24 hours before the injection of PB in MK-801 pretreatment group, and nimodipine after the injection of PB in nimodipine treatment group. The specific binding of NMDA receptor was measured with -MK-801 in the neuronal membrane of cerebral cortex. Results: The levels of water content and EB content of brain tissues, and i in the neuronal synaptosomes increased more significantly in the PB-injected cerebral hemisphere in the PB group than those of normal control group and sham-operated control group (P< 0.05). The water content and i increased with the duration of infectious brain edema. Nimodipine administered after the injection of PB could significantly decrease the water content, EB and i (P< 0.05). MK-801 could significantly decrease the water content, EB and i in 4 h and 24 h groups (P< 0.05). The Kd values were 30.5 nmol/L ±3.0 nmol/L and 42.1 nmol/L ±4.2 nmol/L in PB group and NS group respectively (P< 0.05), and Bmax were 0.606 pmol/mg.pro ±0.087 pmol/mg.pro and 0.623 pmol/mg.pro �

关 键 词：Pertussis toxins Brain edema NIMODIPINE MK-801 Rat 

分 类 号：R651[医药卫生—外科学]