Trigonelline protects the cardiocyte from hydrogen peroxide induced apoptosis in H9c2 cells  

作　　者：Soundharrajan Ilavenil Da Hye Kim Young-Il Jeong Mariadhas Valan Arasu Mayakrishnan Vijayakumar Ponnuraj Nagendra Prabhu Srisesharam Srigopalram Ki Choon Choi 

机构地区：[1]Grassland and Forage division,National Institute of Animal Science,RDA [2]The United Graduate School of Agricultural Sciences,Tottori University [3]School of Medicine,Pusan National University [4]Department of Botany and Microbiology,Addiriyah Chair for Environmental Studies,College of Science,King Saud University [5]Department of Animal Sciences,University of Illinois [6]Department of Horticulture,Sunchon National University

出　　处：《Asian Pacific Journal of Tropical Medicine》2015年第4期263-268,共6页亚太热带医药杂志（英文版）

摘　　要：Objective:To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline(TG)for reducing the H_2O_2induced toxicity in H9c2 cells.Methods:Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX kit.RNA extraction and cDNA synthesized according to the kit manufacture protocol.Apoptosis was measured by the Flowcytometry,general PCR and qPCR.Results:It was found that the TG significantly rescued the morphology of the H9c2 cells.Treatment of cells with TG attenuated H_2O_2 induced cell deaths and improved the antioxidant activity.In addition,TG regulated the apoptotic gene caspase-3,caspase-9 and anti-apoptotic gene Bcl-2.Bcl-XL during H_2O_2 induced oxidative stress in H9c2 cells.These results were comparable with quercetin treatment.For evident,flow cytometer results also confirmed the TG significantly reduced the H_2O_2 induced necrosis and apoptosis in H9c2 cells.However,further increment of TG concentration against H_2O_2 could induce the necrosis and apoptosis along with H_2O_2.Conclusions:It is suggested that less than 125μM of TG could protect the cells from H_2O_2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL expression.Therefore,we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.Objective:To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline（TG）for reducing the H2O2induced toxicity in H9c2 cells.Methods:Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX kit.RNA extraction and cDNA synthesized according to the kit manufacture protocol.Apoptosis was measured by the Flowcytometry,general PCR and qPCR.Results:It was found that the TG significantly rescued the morphology of the H9c2 cells.Treatment of cells with TG attenuated H2O2 induced cell deaths and improved the antioxidant activity.In addition,TG regulated the apoptotic gene caspase-3,caspase-9 and anti-apoptotic gene Bcl-2.Bcl-XL during H2O2 induced oxidative stress in H9c2 cells.These results were comparable with quercetin treatment.For evident,flow cytometer results also confirmed the TG significantly reduced the H2O2 induced necrosis and apoptosis in H9c2 cells.However,further increment of TG concentration against H2O2 could induce the necrosis and apoptosis along with H2O2.Conclusions:It is suggested that less than 125μM of TG could protect the cells from H2O2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL expression.Therefore,we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.

关 键 词：TRIGONELLINE H9C2 cells ANTIOXIDANT Apoptosis CARDIOVASCULAR diseases 

分 类 号：R285[医药卫生—中药学]