High prevalence of multidrug-resistance in Acinetobacter baumannii and dissemination of carbapenemase-encoding genes bla_(OXA-23-like),bla_(OXA-24-like)and bla_(NDM-1) in Algiers hospitals  

作　　者：Khadidja Khorsi Yamina Messai Moufida Hamidi Houria Ammari Rabah Bakour 

机构地区：[1]Laboratory of Cellular and Molecular Biology,Faculty of Biological Sciences,University of Sciences and Technology Houari Boumediene,P.B.32 El-Alia,Bab-Ezzouar,16111 Algiers,Algeria [2]Hospital Salim Zemirli,Route de Baraki.BP N'71,El Harrach 16000,Algiers,Algeria [3]University Hospital Isaad Hassani.Rue Ibrahim Hadjeras,Beni Messous 16206,Algiers,Algeria

出　　处：《Asian Pacific Journal of Tropical Medicine》2015年第6期439-447,共9页亚太热带医药杂志（英文版）

基　　金：supported by grants from National Fund for the Research and National Agency for the Development of Research in Health(Algeria)

摘　　要：Objective:To assess and characterize antibiotic resistance in Acinetobacter baumannii strains recovered from 5 health-care facilities in Algiers.Methods:Antibiotic susceptibility testing was performed by agar diffusion and agar dilution methods,resistance genes were identified by PCR and sequencing,and molecular typing of isolates was carried out by enterobacterial repetitive intergenic consensus-PCR(ERIC-PCR).Results:Among 125 tested isolates,117(93.6% ) were multidrug-resistant.of which 94(75.2% ) were imipenem resistant.The bla_(ADC)and bla_(OXA-51-like) genes were detected in all isolates,in association with ISAba I sequence in 84% and 8% (imipenem resistant) of isolates,respectively.The bla_(OXA-23-like) and bla_(OXA-24-like)carbapenemase genes were delected in 67.02% and 20.21% of imipenem-resistant isolates,respectively.The bla_(OXA-23-like) gene is linked to ISAba1 or ISAba4 elements.The metallo-β-lactamase NDM-1 gene was found in 10(10.6% ) imipenem-resisianl strains from three hospitals,it is linked to ISAba125 clement in nine strains.Extended spectrum β-lactamases production was not detected.Imipenem and cefotaxime resistance phenolypes could not be transferred to Escherichia coli by conjugation.Outer membrane protein CarO gene was not delected in four imipenem-resisianl isolates.The aac(6')-1b.sul1,sul2,tetA and tetB genes were present in 5.31% .36.17% .77.65% .1.06% and 65.92% of strains,respectively.Class 1 integrons were detected in 23.4% strains.KRIC-PCR typing showed a genetic diversity among bla_(OXA-23-like) and bla_(OXA-24-like) positive strains,while clonality was observed among bla_(NDM-1)positives.Conclusions:This study highlighted the high prevalence of imipenem resistance in Acinetobacter baumannii in Algiers hospitals mediated mainly by bla_(OXA-23-like),bla_(OXA-24-like),and bla_(NDM-1) genes.Objective:To assess and characterize antibiotic resistance in Acinetobacter baumannii strains recovered from 5 health-care facilities in Algiers.Methods:Antibiotic susceptibility testing was performed by agar diffusion and agar dilution methods,resistance genes were identified by PCR and sequencing,and molecular typing of isolates was carried out by enterobacterial repetitive intergenic consensus-PCR（ERIC-PCR）.Results:Among 125 tested isolates,117（93.6% ） were multidrug-resistant.of which 94（75.2% ） were imipenem resistant.The blaADCand blaOXA-51-like genes were detected in all isolates,in association with ISAba I sequence in 84% and 8% （imipenem resistant） of isolates,respectively.The blaOXA-23-like and blaOXA-24-likecarbapenemase genes were delected in 67.02% and 20.21% of imipenem-resistant isolates,respectively.The blaOXA-23-like gene is linked to ISAba1 or ISAba4 elements.The metallo-β-lactamase NDM-1 gene was found in 10（10.6% ） imipenem-resisianl strains from three hospitals,it is linked to ISAba125 clement in nine strains.Extended spectrum β-lactamases production was not detected.Imipenem and cefotaxime resistance phenolypes could not be transferred to Escherichia coli by conjugation.Outer membrane protein CarO gene was not delected in four imipenem-resisianl isolates.The aac（6’）-1b.sul1,sul2,tetA and tetB genes were present in 5.31% .36.17% .77.65% .1.06% and 65.92% of strains,respectively.Class 1 integrons were detected in 23.4% strains.KRIC-PCR typing showed a genetic diversity among blaOXA-23-like and blaOXA-24-like positive strains,while clonality was observed among blaNDM-1positives.Conclusions:This study highlighted the high prevalence of imipenem resistance in Acinetobacter baumannii in Algiers hospitals mediated mainly by blaOXA-23-like,blaOXA-24-like,and blaNDM-1 genes.

关 键 词：Acinetobacter baumannii MULTIDRUG-RESISTANCE CARBAPENEMASE bla_(OXA-23-like) bla_(OXA 24 like) bla_(NDM-1) carO Hospital Algiers 

分 类 号：R446.5[医药卫生—诊断学]