Aβ对原代培养海马神经元NO、NOS和LDH影响及bFGF保护作用  

Protective Effect of bFGF on NO, NOS and LDH Concentration Changes in Cultured Hippocampal Neurons Exposed to Amyloid-β Protein

作　　者：李积荣[1] 苗建亭[1] 宿长军[1] 李柱一[1] 林宏[1] 车宇[1] 

机构地区：[1]第四军医大学唐都医院神经内科,陕西西安710038

出　　处：《中国神经免疫学和神经病学杂志》2003年第2期79-82,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金："十五"全军医药卫生科研基金资助项目 (0 1Q112 ) ;第四军医大学科技创新工程基金资助项目 (CX0 1A0 18)

摘　　要：目的　探讨β-淀粉样蛋白 (amyloid-beta protein,Aβ)对原代培养海马神经元一氧化氮 (NO)、一氧化氮合酶 (NOS)和乳酸脱氢酶 (LDH)的影响及碱性成纤维细胞生长因子 (b FGF)的保护作用。方法　通过测定原代培养新生大鼠海马神经元培养液中 NO、NOS及 LDH的含量变化 ,观察 Aβ1 -40对神经元的损伤及 b FGF的保护作用。结果　Aβ1 -40作用于海马神经元 2 4h后 ,培养上清液中 NO、NOS含量增加 ,LDH活性升高 ,与对照组比较差异有显著性 (P<0 .0 5 ,P<0 .0 1 )。经 b FGF预处理的海马神经元暴露于 Aβ1 -40 2 4h后 ,NO、NOS含量及 LDH活性均降低 ,且与损伤组比较差异有显著性 (P <0 .0 5 )。结论　 b FGF对 Aβ诱导海马神经元损伤具有一定的保护作用。Objective To investigate the changes of NO, NOS and LDH of cultured hippocampal neurons induced by amyloid β protein (Aβ) and the protective effect of bFGF.Methods Primitive hippocampal neurons were culctured. Concentrations of NO, NOS and LDH in cultured supernatant were measured after 24 h when cultured neurons exposed to Aβ and bFGF.Results Content of NO, NOS and LDH in cultured supernatant increased obviously when hippocampal neurons were exposed to Aβ for 24 h than that of control group. After pretreated with bFGF for 24 h and then exposed to Aβ, NO, NOS and LDH levels were lowered markedly. Conclusions Aβ might increase contents of NO, NOS and LDH of supern atant of cultured neurons. bFGF could protect the damage induced by Aβ.

关 键 词：Β-淀粉样蛋白 海马 神经元 一氧化氮 一氧化氮合酶 乳酸脱氢酶 碱性成纤维细胞生长因子 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]