急性缺血性脑卒中患者外周血白细胞CD11b、CD18表达变化研究  

The Expression of CD11b and CD18 on the Leukocytes of Peripheral Blood in Patients with Acute Ischemic Stroke

作　　者：唐方[1] 陈槐卿[2] 徐朝义[3] 张峻梅[1] 高励[3] 刘凯[1] 

机构地区：[1]成都市第三人民医院检验科,四川成都610031 [2]华西医科大学生物医学工程研究室,四川成都610061 [3]成都市第三人民医院神经内科,四川成都610031

出　　处：《中国神经免疫学和神经病学杂志》2003年第2期119-122,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金资助项目 (3 9670 2 0 2 )

摘　　要：目的　探讨急性脑卒中患者外周血白细胞黏附分子 CD1 1 b、CD1 8的表达 ,以及其在急性缺血性脑损伤发生、发展中的作用。方法　采用流式细胞术 ,用单克隆抗体标记定量测定 3 2例急性脑卒中患者发作 2 4h内外周血白细胞 CD1 1 b、CD1 8的表达量 ,以平均荧光强度 (MFI)的大小来表示 CD1 1 b、CD1 8的相对含量 ,在发病72 h、7d后再分别测定 1次。以 40例健康者作对照。结果　发病 2 4h内患者组白细胞表面黏附分子 CD1 1 b、CD1 8的表达显著高于对照组 (P <0 .0 1 ) ;在发病 72 h内患者组 CD1 1 b、CD1 8的表达逐渐降低 ,但仍高于对照组 (P<0 .0 5 ) ;发病 7d后 ,其结果与对照组相比差异无显著性 (P >0 .0 5 )。结论　急性脑卒中发病时 ,白细胞被激活 ,黏附分子 CD1 1 b、CD1 8表达上调 ,介导白细胞与内皮细胞黏附增强 ,可能会加重缺血后迟发性神经元死亡的病理生理过程。Objective To study the role of expression of adhesion molecules CD11b, CD18 on the leukocytes of peripheral blood in patients with acute cerebral infarction.Methods Using immunofluorescence phenotyping and flow cytometry, the expression of leukocytes adhesion molecules CD11b, CD18 in peripheral blood were measured within 24 h after onset of acute cerebral infarction of 32 patients. Follow up measurements were performed at 72 h and 7 d after ictus.Results CD11b, CD18 immunofluorescence intensity was significantly increased on the leukocytes within 24 h and 72 h after onset in patients with ischemic stroke compared with the normal age matched control group. Follow up measurement of CD11b, CD18 within 7 d after onset revealed normal results as found in the control group.Conclusions These results indicate that the expression of CD11b,CD18 on leukocytes increases in acute ischemic damage, thus promoting adhesion of leukocytes with endothelial cells, which may result in aggravate post ischemic delayed neuronal death.

关 键 词：急性缺血性脑卒中 外周血 白细胞 CD11B CD18 流式细胞术 单克隆抗体 迟发性神经元死亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]