玻璃纤维诱导的转化细胞活化癌基因的研究  

The Observation of Oncogene Activation in Transformed Cells Induced by Glass Fibers

作　　者：高宏光[1] 王国荃[2] 翁烔满 黄文溶 

机构地区：[1]广州医学院化学致癌研究所,广州510182 [2]新疆医学院环境卫生学教研室,乌鲁木齐830054 [3]美国国家职业安全与卫生研究所,morgantownwv26505

出　　处：《卫生毒理学杂志》1992年第4期261-263,共3页Journal of Health Toxicology

摘　　要：用玻璃纤维处理BALB/C3T3细胞,使细胞发生转化,从转化细胞中抽提DNA用以转染NIH3T3细胞,结果被转染的细胞亦发生转化。用^(32)P标记的Ha-ras癌基因作探针,通过斑点杂交,征明玻璃纤维诱发的BALB/3T3转化细胞中,Ha—ras的原癌基因被激活。Studies have been performed in our laboratory to determine whether three glass fibers, JM 100, owens corning AAA-10, and owens corning general building insulation fibers can induce morphological transformation in BALB/c3T3 cells. DNA from transformation cells were isolated and analyzed for oncogene activation. The results showed that reaction in vitro with activated oncogene, c-Ha-ras, when the transformed cell DNA was introduced into NIH3T3 cells, c-Ha-ras oncogene were expressed in the transformed cells and formed the morphological tra-nsformation of NIH3T3 cells. These characterization for the transformation indicated that c-Ha-ras oncogene were activated in the NIH3T3 cells and performed malignant phenotype.

关 键 词：玻璃纤维 转化 转染 癌基因 

分 类 号：R730.231.1[医药卫生—肿瘤]