PDHA1基因敲除对人食管癌细胞生物学性状影响  

Effect of PDHA1 gene knockout on biological phenotypes of esophageal cancer cell line

作　　者：钟亚莉[1] 李健[2] 赵治国[1] 索振河[3] 

机构地区：[1]郑州大学第二附属医院消化内科,郑州450017 [2]河南省人民医院消化内科,郑州450003 [3]郑州大学第一医附属医院肿瘤内科,郑州450052

出　　处：《慢性病学杂志》2015年第5期532-534,共3页Chronic Pathematology Journal

基　　金：国家自然科学基金面上项目(81272824)

摘　　要：目的研究PDHA1基因敲除对人食管癌细胞的影响,以了解PDHA1基因表达下降在人食管癌复发转移中的作用。方法采用细胞转染TALEN质粒对的方法,利用食管癌细胞系EC9706构建PDHA1基因敲除细胞株,比较其与亲本细胞在表型差别。结果 PDHA1基因敲除后,PDHA1蛋白表达水平明显下降,细胞表现出生长速度减慢;但在体外细胞移动实验中表现出穿膜能力明显增强,穿过滤膜的细胞数(45±8)个,而亲本细胞仅有(24±6)个,差异有统计学意义(P<0.05)。结论成功建立PDHA1基因敲除的稳定细胞系,PDHA1基因敲除后食管癌细胞增殖受到抑制,但是细胞体外运动能力增强,提示PDHA1基因表达下降可能在人食管癌复发、侵袭转移中有重要的意义。Objective To observe the effect of PDHA1 gene knockout on the esophageal cancer cell lines, in order to analyze Warburg effect in malignancy development of human esophageal cancer. Methods We developed the PDHA1 gene knockout cell line and then investigated the phenotypic changes of EC9706. Results With PDHA1 gene knockout, the PDHA1 protein expression downregulated and the cell exhibited slower proliferation. In the transwell assay, migrated cell number of PDHA1 KO cell and parental cell number were 45±8 cells and 24±6 cells(P<0.05) respectively. Conclusions PDHA1 gene knockout cell line has been established. The proliferation of PDHA1 KO cell was inhibited. However,cell migration ability enhanced. Our study indicates that downregulated or deleted PDHA1 may contribute to relapse and metastasis of human esophagus cancer.

关 键 词：PDHA1 食管癌 转移 

分 类 号：R735.1[医药卫生—肿瘤]