昆明山海棠碱诱导Jurkat淋巴瘤细胞株的PARP酶剪切和caspase-3激活  

Tripterygium Hypoglaucum (Levl) Hutch alkaloids induce PARP cleavage and caspase-3 activation in Jurkat T lymphoma cells

作　　者：杨录军[1] 敖琳[1] Nuesse Michael 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理学教研室 [2]GSF-National Research Center for Environment and Health,Neuherberg,Germany,D85764

出　　处：《第三军医大学学报》2003年第17期1508-1510,共3页Journal of Third Military Medical University

基　　金：德国联邦教育科学研究技术部 (BMBF)基金资助项目 ;国家自然科学基金资助项目 (C30 10 0 2 4 1) ;第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)

摘　　要：目的　探讨昆明山海棠碱诱导淋巴瘤细胞凋亡过程中caspase 3和PARP酶的作用 ,以了解THH碱诱导细胞凋亡的机制。方法　THH碱诱导JurkatT淋巴瘤细胞后 ,FAM DEVD FMK荧光标记活化的caspase 3 ,免疫细胞化学方法荧光标记PARP酶剪切后的 89× 10 3片段 ,均以流式细胞术检测。结果　THH碱能有效诱导caspase 3激活和PARP酶剪切。结论　提示在THH碱能诱导JurkatT淋巴瘤细胞凋亡过程中caspase 3和PARP酶参与调控 ,它们可能抑制了受损伤DNA的修复 。Objective To explore the roles of caspase 3 and poly (ADP ribose) polymerase (PARP) in the apoptotic process of Jurkat T lymphoma cells induced by Tripterygium Hypoglaucum (Levl) Hutch alkaloids to understand the mechanisms of the apoptosis. Methods After induction by the alkaloids, the activated caspase 3 was fluorescenc labelled with FAM DEVD FMK and PARP cleavage was detected immunocytochemically using antibody which can recognize its 89×10 3 fragment. Then determination by flow cytometry was performed. Results The THH alkaloids could effectively induce PARP cleavage as well as activation of caspase 3. Conclusion We conclude that caspase 3 and PARP take part in the modulation of apoptosis induced by THH alkaloids, resulting in DNA fragmentation by means of inhibition of the repair of the damaged DNA.

关 键 词：昆明山海棠 细胞凋亡 PARP DNA修复 caspase—3 

分 类 号：R282.71[医药卫生—中药学] R394.6[医药卫生—中医学]