犬肺癌模型制作与支气管动脉造影  

Establishment and bronchial arteriography of the models of canine lung cancer

作　　者：曹喜才[1] 刘勇[2] 徐乃勋[3] 王晓东[1] 马卫军[3] 杨海贤[4] 白景文[4] 李斌[1] 石建光[1] 李卫东 刘淑萍 刘安甫 

机构地区：[1]天津医科大学总医院放射科,300052 [2]天津医科大学 [3]天津市胸科医院 [4]天津医科大学电镜中心 [5]解放军第二五四医院

出　　处：《中华放射学杂志》2003年第10期877-881,共5页Chinese Journal of Radiology

基　　金：天津市自然科学基金课题 (983 60 85 11)

摘　　要：目的　探讨犬肺癌模型的制作与支气管动脉造影。为肺癌进一步诊断和治疗创造条件。方法　 2 2只犬经气管插管由同轴微导管精确定位 ,向右肺膈叶灌注甲基胆蒽 (MCA)、二乙基亚硝胺 (DEN)和超液化碘油混悬液 3ml。随机分为 5组 ,A(4只 )、B(4只 )、C(4只 )、D(5只 )、E组(5只 ) ;分别观察至 1、3、6、12、18个月时处死 ,观察不同时期病理和影像学变化。对观察 18个月的5只犬行支气管动脉造影。结果　 1个月时可见细支气管周围炎 ,肺不张 ;3个月后逐渐形成慢性肉芽肿性炎症 ,肺组织纤维化 ;6～ 12个月时出现细支气管内干细胞增生 ,肺组织不典型增生 ;18个月时分化为鳞癌和细支气管肺泡癌。 18个月诱导 5只犬的肺癌及癌前相关损害 ,成功 4只。 5只犬支气管动脉造影检查 ,3只支气管动脉向肿瘤供血。结论　经气管插管由同轴微导管精确定位灌注MCA、DEN和超液化碘油混悬液诱导犬肺癌模型 ,定位准确 ,诱导成功率高 ;证实犬肺癌模型由支气管动脉供血 ;支气管动脉造影对肺癌早期诊断具有一定意义。犬肺癌模型对肺癌诊断与治疗 。Objective To investigate the establishment and bronchial arteriography of the models of canine lung cancer, and to facilitate further diagnosis and treatment of lung cancer. Methods Twenty-two dogs were respectively administrated with 3 ml suspension of lipiodol-ultrafluid mixed with 3-methylcholanthrene (MCA) and diethylnitrosamine (DEN). The suspension was injected into the diaphragmic lobe of right lung with the co-axial catheter through endotracheal intubation. The dogs were randomly divided into 5 groups, group A (4 dogs), B (4), C (4), D (5), and E (5), which were sacrificed after observation for 1, 3, 6, 12, and 18 months, respectively. Then the image analysis and histopathologic examinations were carried out at different period. 5 dogs in group E were examined by bronchial arteriography. Results Peribronchiolitis and atelectasis appeared at early stage (one month). Chronic granulomatous inflammation and fibrosis of lung tissue were gradually formed after 3 months. Proliferation of stem cells in bronchioles and atypical hyperplasia were found from 6 to 12 months. At last, the squamous carcinoma and bronchioloalveolar carcinoma were induced after 18 months. The lung cancer and precancerous lesion were found in 4 of 5 dogs at the 18 th month. The bronchial arteriography in the 5 dogs showed that 3 bronchial arteries were found as the supplying blood artery to the tumor. Conclusion The suspension of lipiodol-ultrafluid mixed with MCA and DEN was exactly injected with the co-axial catheter through endotracheal intubation to establish the models of canine lung cancer. The inducible rate of the method was high and the location of lung cancer was accurate. It was affirmed that the blood supply artery of canine lung cancer was bronchial artery. Bronchial arteriography was of momentous significance to the diagnosis of early lung cancer. The establishment of the models of canine lung cancer was significant in the diagnosis and treatment of lung cancer. The models were advantageous to further study of intervent

关 键 词：犬 肺癌 动物模型 制作 支气管动脉造影 

分 类 号：R734.2[医药卫生—肿瘤] R73-3[医药卫生—临床医学]