野生、栽培大豆愈伤组织耐盐性与植株盐胁迫下丙二醛(MDA)含量的关系  

THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEAN AND MDA CONTENT OF PLANT GROWN IN SALTY SOIL

作　　者：乔亚科[1] 李桂兰[1] 王文颇[1] 高书国[1] 毕艳娟[1] 景燕[1] 

机构地区：[1]河北职业技术师范学院农学系,昌黎066600

出　　处：《大豆科学》2003年第2期127-131,共5页Soybean Science

基　　金：河北省科技厅项目 ( 992 30 313)。

摘　　要：以野生、栽培大豆共 14份材料的下胚轴作为外植体诱导愈伤组织 ,在含不同浓度NaCl的培养基上鉴定愈伤组织的耐盐性 ,NaCl浓度 (w/v)分别为 0 % (CK ) ,0 .1% ,0 .2 % ,0 .3% ,0 .4 % ,0 .5 % ,0 .6% ,0 .7% ,0 .8% ;14份材料的幼苗在NaCl为 0 .3%的盐土环境中生长 2 5d后 ,测其幼苗的丙二醛 (MDA)含量。试验结果表明 :愈伤组织水平上 ,栽培大豆品种中黄 4、通农 5、吉林 35耐盐性较强 ,不同材料之间愈伤组织的耐盐性有明显差异 ,耐盐性强的可在 0 .8%NaCl浓度下生长 ,耐盐性弱的只能忍受 0 .4 %NaCl浓度 ;本试验确定大豆愈伤组织耐盐性鉴定适宜的浓度为 0 .6%。在盐胁迫下 ,植株MDA含量在 0 .3%NaCl条件下均比在CK土壤中高 ,但随胁迫时间的延长而呈现不同的变化趋势。综合大豆植株MDA含量的变化和愈伤组织的耐盐性之间 ,并未发现相关性。The 14 wild soybeans and cultivated soybeans were studied in this experiment. Hypocotyls were used as explants. Callus was induced at MS+2,4-D2.0mg/L+6-BA0.5mg/L + sucrose 3% + agar 0.8% . The salt tolerance of callus was observed on different medium containing 0(as contrast),0.1%, 0.2% ,0.3%, 0.4% ,0.5%,0.6%,0.7%,0.8% NaCl. The experimental materials were planted also in soil pond containing 0.0% ?0.3%NaCl. The results showed that salt tolerance of callus from Zhonghuang 4,Tongnong5 and Jilin35 was stronger. Among different materials, the difference of salt tolerance was remarkable. The higher salt tolerance materials could grow well on medium containing 0.8%NaCl , the lower salt tolerance materials could only grow on medium containing 0.4%NaCl. Suitable concentration for identifing salt tolerance of soybean callus was 0 6% NaCl in this experiment MDA content of plants in 0.3% NaCl was higher than that in control. MDA content of plants grown on 0.4% NaCl soil pond at different time appeared different varies with the increase of salt stress days.The correlation between MDA content and callus salt tolerance was not found in this paper.

关 键 词：野生大豆 栽培大豆 愈伤组织 耐盐性 MDA含量 

分 类 号：S565.1[农业科学—作物学]