黄芪多糖对丝裂霉素C(MMC)致骨髓抑制小鼠骨髓及脾脏造血祖细胞的生成作用的影响  

Effects of astragalus polysaccharide (APS) on murine bone marrow and spleen progenitor cells in MMC-induced myleosuppression

作　　者：夏星 Dao Nguyen 

机构地区：[1]美国泛华医药公司,北京100020

出　　处：《中国药理学通报》2003年第7期812-814,共3页Chinese Pharmacological Bulletin

摘　　要：目的　研究黄芪多糖 (APS)对MMC致骨髓抑制小鼠的骨髓和脾造血祖细胞生长的影响。方法　d 0腹腔注射丝裂霉素C(MMC) 7mg·kg- 1 ,APS皮下注射 1 0 0mg·kg- 1·d- 1 ,给药方案分 3种 :① 0～ 4d ,共 5d ;② 0～ 1 1d ,共 1 2d ;③ 1 2d给药 ,3wk内给完 ,用造血细胞集落培养法观察APS的药理作用。结果　在d 3时 ,APS治疗组对MMC致骨髓抑制小鼠骨髓CFU C数目增加了 3倍 ,分别为 1 870±40 /股骨和 62 4 0± 1 1 0 /股骨 ,从d 3～d 1 8,APS治疗组的骨髓CFU C均高于模型组。在给MMC后d 1 4之前APS对脾CFU C的生长没有影响 ,APS在d 1 4和d 1 8时APS有刺激脾造血祖细胞增殖作用 (分别高 3倍和 2倍 )。AIM To observe the effect of APS on bone marrow and spleen progenitor cells in MMC induced myelosuppression in mice. MEHTODS MMC 7 mg·kg -1 was injected ip on day 0, APS 100 mg·kg -1 ·d -1 was given sc in 3 regimens ① on day 0～4, ② on day 0～11,③ 12 doses in 3 weeks. RESULT APS increased the number of bone marrow progenitor cells(CFU C) of MMC treated mice 3 fold, from 1 870 ±40 per femur to 6 240 ±110 per femur on day 3.APS treatment resulted in a higher level of bone marrow CFU C at all time points from day 3 to day 18, in comparison with the control group. APS also significantly stimulated the proliferation of spleen progenitor cells on day 14 (3 fold of control) and day 18(2 fold of control) after MMC treatment, however APS had no effect on spleen progenitor cells before day 14. CONCLUSION These results demonstrate that APS enhances the proliferation and maturation of the progenitors of peripheral blood cells in MMC treated mice.

关 键 词：黄芪多糖 MMC 造血组细胞 

分 类 号：R-332[医药卫生] R284.1