苦马豆素对人食道癌Eca-109细胞体外生长的抑制试验  

Inhibition Effect of Swainsonine on Human Esophagus Carcinoma Cell Line Eca-109 in vitro

作　　者：陈绍淑[1] 赵宝玉[1] 莫重辉[2] 童德文[1] 王立红[3] 王凯[4] 曹光荣[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100 [2]青海大学农牧学院,青海西宁810003 [3]天津市奶牛发展中心,天津300221 [4]佛山科技学院动医系,广东佛山528231

出　　处：《动物医学进展》2003年第3期91-92,共2页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目 (39770 571 )

摘　　要：探讨苦马豆素 (SW)对人食道癌 Eca-10 9细胞生长的抑制作用 ,以揭示 SW治疗食道癌的作用机制。选用不同剂量的 SW与食道癌Eca- 10 9细胞共同培养不同时间后 ,用 MTT法观察 SW对食道癌 Eca- 10 9细胞生长的抑制作用 ;细胞 DNA经特异性荧光染色后 ,用流式细胞仪分析细胞周期变化。结果 :SW对食道癌Eca- 10 9细胞生长的半数抑制浓度 IC50 <2 .5μg/ m L;细胞周期分析 ,Eca- 10 9细胞 G1期细胞增多 ,S期细胞减少。结果表明 :SW可通过抑制人食道癌 Eca- 10 9细胞系生长达到抑制肿瘤的目的。We investigated the inhibition effect of swainsonine(SW) on human esophagas carcinoma cell line to explorie mechanism of treatment of esophagus carcinoma.Methods A human esophagus carcinoma cell line Eca 109 was cultured in full medium with variable doses of SW for different hours. The inhibition effect of SW on esophagus carcinoma cell line Eca 109 was examined with MTT. Cell cycle analysis was performed on flow cytometry (FCM).Results The IC 50 of SW on esophagus carcinoma cell Eca 109 was lower than 2.5 μg/mL.The analysis of cell cycle indicated that the number of the cells was increased in the G1 phase, but decreased in the S phase of the cell cycle.Conclusion SW may inhibit the growth of human esophagus carcinoma cell line Eca 109,which led to the death of the cancer cell.

关 键 词：苦马豆素 人食道癌 ECA-109细胞 体外生长 抑制试验 

分 类 号：R735.1[医药卫生—肿瘤]