褪黑素抑制实验性老化大鼠海马注射Aβ_(1-40)毒性作用  

The preventive effects of melatonin against damage induced by amyloid beta-peptide 1-40 in senile rats

作　　者：谢桂琴[1] 孙秀兰[1] 田苏平[1] 陈启盛[1] 

机构地区：[1]南京医科大学生理系,江苏南京210029

出　　处：《中国神经科学杂志》2003年第3期167-170,共4页

基　　金：江苏省自然科学基金资助项目 (KJB3 10 0 0 4)

摘　　要：目的 :观察褪黑素 (melatonin ,MT)对实验性老年性痴呆大鼠的防治作用。 方法 :连续 6周腹腔内注射D 半乳糖 ,双侧海马内注射Aβ1 4 0 造成AD模型。以Y 型迷宫、一次性被动回避实验检测大鼠的学习记忆行为 ,测定大脑皮层脂褐素含量以及海马线粒体膜流动性、SOD活性、MDA含量 ,电镜观察海马超微结构。结果 :褪黑素可显著改善痴呆大鼠的学习记忆障碍 ,明显逆转海马SOD活性、MDA含量及皮层脂褐素含量相对其正常水平的增高 ,阻抑海马神经元的变性及凋亡。 结论 :褪黑素可能通过影响自由基水平对实验性老年性痴呆大鼠有一定的防治作用。Objective:To study the protective and therapeutic effects of melatonin on the model rats of Alzheimer's disease(AD). Methods:D galactose was intraperitoneally injected in rats for 6 weeks and Aβ 1 40 was injected into both hippocampus to make AD models. Melatonin was intraperitoneally injected in rats for 4 weeks.Y maze test and step though test were used to determine the ability of learning and memory. The content of lipofuscin in the central cortex, the viscous coefficient of mitochondrial membrane, the activity of superoxide dismutase and the content of malondialdehyde in both sides of hippocampus were measured. And the apoptosis of hippocampus neurons was detected with transmission electron microscopy(TEM). Results :The melatonin as an antioxidant significantly improved learning and memory deficits in the rats with AD and attenuated the elevation of SOD, MDA, the viscous coefficient and lipofuscin to their normal levels, and it also showed the protective effects against apoptosis. Conclusion:Melatonin had preventive and therapeutic effects on rats with AD probably by affecting the free radical levels in rats.

关 键 词：褪黑素 实验 大鼠 AΒ1-40 神经毒性 老年性痴呆 海马内注射 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]