喉鳞状细胞癌微血管密度和层粘连蛋白表达与颈淋巴结转移的关系  

Relationship Between the Microvessel Density and Expression of LN and Cervical Node Metostasis of Laryngeal Squamous Cell Cancer

作　　者：谢晓凤[1] 王建中[1] 吴钦苏[1] 周旭[1] 罗雪梅[1] 常荣先[1] 

机构地区：[1]复旦大学附属中山医院耳鼻喉科,上海200032

出　　处：《复旦学报（医学版）》2003年第6期585-587,共3页Fudan University Journal of Medical Sciences

摘　　要：目的 探讨喉鳞癌中微血管密度(microvessel density,MVD)和层粘连蛋白(laminin,LN)表达与颈淋巴结转移的关系。方法 应用CD34和LN抗体,采用免疫组织化学(Envision)染色方法对54例喉鳞癌组织中微血管密度及LN表达进行了检测。结果 (1)喉鳞癌组织中MVD与颈淋巴结转移相关,淋巴结转移组的MVD高于非转移组(P<0.05);MVD与喉鳞癌临床分期(TNM)和病理分级无关(P>0.05)。(2)LN表达与喉鳞癌颈淋巴结转移相关,有淋巴结转移组LN表达率高于无淋巴结转移组(P<0.05);LN表达与肿瘤组织病理分级和临床分期(TNM)无关(P>0.05)。(3)喉鳞癌组织中MVD与LN表达无关(P>0.05)。结论 喉鳞癌中MVD和LN表达可作为预测颈淋巴结转移的指标。Purpose: To study the relationship between the microvessel density (MVD) and expression of laminin (LN) and cervical lymph node metostasis of laryngeal squamous cell cancer (LSCC). Methods: Immunohistochemical analyses (Envision method) using antibodies against CD34 and LN was carried out on archival specimens of 54 human laryngeal squamous cell cancer. Results: (1) MVD was higher in LSCC with positive lymph node than that with negative lymph node (P 0.05). Conclusions: MVD and LN expression were helpful in predicting LSCC patients at risk for cervical lymph node metastasis.

关 键 词：喉鳞状细胞癌 微血管密度 层粘连蛋白 颈淋巴结转移 免疫组织化学 检测 

分 类 号：R739.65[医药卫生—肿瘤] R730.4[医药卫生—临床医学]